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N3129

Sigma-Aldrich

4-硝基苯磷酸盐 双环己铵盐

phosphatase substrate, chromogenic, ≥97% anhydrous basis, powder

别名:

p-Nitrophenyl phosphate bis(cyclohexylammonium) salt, PNPP

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About This Item

线性分子式:
O2NC6H4OP(O)(O-)2 · 2C6H11NH3+
CAS号:
52483-84-8
分子量:
417.44
Beilstein:
6553276
EC號碼:
257-949-0
MDL號碼:
MFCD00013173
分類程式碼代碼:
12352204
PubChem物質ID:
24897623
NACRES:
NA.83

product name

4-硝基苯磷酸盐 双环己铵盐, phosphatase substrate

品質等級

300

化驗

≥97% anhydrous basis

形狀

powder

溶解度

water: 10 mg/mL, clear, colorless to faintly yellow

儲存溫度

−20°C

SMILES 字串

NC1CCCCC1.NC2CCCCC2.OP(O)(=O)Oc3ccc(cc3)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2

InChI 密鑰

KXNNUYCXODNFBD-UHFFFAOYSA-N

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相关类别

應用

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

生化／生理作用

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Brittany R Jenkins et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(6), e21551-e21551 (2021-05-28)
Intestinal epithelial cells (IEC) are crucial for maintaining proper digestion and overall homeostasis of the gut mucosa. IEC proliferation and differentiation are tightly regulated by well described pathways, however, relatively little is known about how cytokines shape these processes. Given
Ziad Dahabreh et al.
World journal of stem cells, 6(4), 497-504 (2014-09-27)
To compare seven commercially available bone graft substitutes (BGS) in terms of these properties and without using any additional biological growth factors. Porcine osteoprogenitor cells were loaded on seven commercially available BGS and allowed to proliferate for one week followed
Leaf abscisic acid accumulation in response to substrate water content: Linking leaf gas exchange regulation with leaf abscisic acid concentration
Bauerle WL, et al.
J. Am. Soc. Hort. Sci., 131(2), 295-301 (2006)
Abscisic acid synthesis in Acer rubrum L. leaves?a vapor-pressure-deficit-mediated response
Bauerle WL, et al.
J. Am. Soc. Hort. Sci., 129(2), 182-187 (2004)
Bjarni Ásgeirsson et al.
Biochemistry and biophysics reports, 24, 100830-100830 (2020-10-27)
Para-nitrophenyl phosphate, the common substrate for alkaline phosphatase (AP), is available as a cyclohexylamine salt. Here, we report that cyclohexylamine is a non-competitive inhibitor of APs. Cyclohexylamine inhibited four different APs. Co-crystallization with the cold-active Vibrio AP (VAP) was performed

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