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一般說明
Solid Phase Reversible Immobilization (SPRI) beads are magnetic beads that can be utilized for DNA purification and size selection. The 5R-PLEX beads are SPRI beads designed to easily separate the desired PCR amplicon from nucleotides, adapters, primers, and primer dimers by efficient purification and size selection. The magnetic beads are provided in a ready to use suspension that enables fast and efficient application even in high throughput applications with automated liquid handling. The beads are formulated with NaCl and high purity PEG as the crowding agent.
應用
- NGS library preparation, including 16S and 18S
- Approved to use in ultrasensitive 5R-Plex (SMURF) library preparation(2,3,4) for degraded samples
- PCR reaction clean up
- Amplicon size selection
- DNA purification and manipulation (replacing elution buffer, concentrating the sample)
特點和優勢
- Ready to use
- Compatible with automated liquid handling
- Easy size selection by changing the volumetric ratio of beads to samples
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
Solid-phase reversible immobilization for the isolation of PCR products.
Nucleic acids research, 23(22), 4742-4743 (1995-11-25)
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The human fetal immune system begins to develop early during gestation; however, factors responsible for fetal immune-priming remain elusive. We explored potential exposure to microbial agents in utero and their contribution toward activation of memory T cells in fetal tissues. We
Stepwise remodeling and subcompartment formation in individual vesicles by three ESCRT-III proteins.
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The endosomal sorting complex required for transport (ESCRT) is a multi-protein machinery involved in several membrane remodeling processes. Different approaches have been used to resolve how ESCRT proteins scission membranes. However, the underlying mechanisms generating membrane deformations are still a
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