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用途
sufficient for 100 colorimetric or fluorometric tests
檢測方法
colorimetric
fluorometric
相關疾病
cancer
儲存溫度
−20°C
基因資訊
human ... PLD1(5337) , PLD2(5338) , PLD3(23646) , PLD4(122618) , PLD5(200150) , PLD6(201164)
mouse ... PLD1(18805) , PLD2(18806) , PLD3(18807) , PLD4(104759) , PLD5(319455) , PLD6(194908)
rat ... PLD1(25096) , PLD2(25097) , PLD3(361527) , PLD4(362792) , PLD5(289270) , PLD6(287366)
一般說明
磷脂酶 D (PLD) 催化甘油磷脂的磷酸二酯键水解生成磷脂酸和胆碱。它被广泛表达，参与免疫功能和癌症抑制。PLD 表达异常与人类癌症相关，如乳腺癌、肾癌、胃癌和结直肠癌。
應用
适于检测生物样品中的磷脂酶 D 的活性。 本试剂盒可用于研究各种化合物对磷脂酶 D 活性的影响。
采用磷脂酶 D (PLD) 活性检测试剂盒测定了小鼠肝脏和肝脏溶酶体中 PLD 活性。
特點和優勢
兼容高通量的操作系统。
適合性
适用于磷脂酶 D 活性的定量测定及药物对磷脂酶 D 代谢影响的评价。
原則
在本试验中，PLD 将磷脂酰胆碱水解为胆碱，使用胆碱氧化酶进行测定，得到比色 (570 nm)/荧光（λex = 530/λem = 585 nm）乘积，与样品中 PLD 活性成正比。单位定义：在试验条件下 (pH 7.4)，1 个单位的 PLD 每分钟催化 1 mmol 胆碱的形成。
訊號詞
Danger
危險聲明
危險分類
Aquatic Chronic 3 - Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
閃點（°F）
188.6 °F - closed cup
閃點（°C）
87 °C - closed cup
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