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![2-脱氧-2-[（7-硝基-2,1,3-苯并恶二唑-4-基）氨基]-D-葡萄糖 ≥97% (HPLC)](/deepweb/assets/sigmaaldrich/product/structures/104/527/40bd5a41-ebc4-484e-a10e-891fecfaea79/640/40bd5a41-ebc4-484e-a10e-891fecfaea79.png)
用途
sufficient for 100 fluorometric tests
檢測方法
fluorometric
相關疾病
endocrinological disorders, diabetes; cancer
儲存溫度
−20°C
一般說明
现在我们可提供全新的葡萄糖摄取检测试剂盒MAK505！葡萄糖是大多数细胞的主要能量来源。葡萄糖的跨细胞膜运输是葡萄糖代谢中的第一个限速步骤。GLUT家族的转运蛋白可促进葡萄糖摄取，而这些蛋白的表达和活性受到多种机制的调控。许多癌细胞的葡萄糖摄取增加，表现出高速率的有氧糖酵解。胰岛素抵抗细胞表现出更少的胰岛素刺激后葡萄糖摄取。
應用
葡萄糖摄取检测试剂盒（荧光法）已可用于测量单核细胞样髓源抑制细胞（M-MDSC）的葡萄糖摄取量。
適合性
适用于检测在96孔微量滴定板中培养的贴壁细胞或悬浮细胞的葡萄糖摄取。
原則
葡萄糖摄取检测试剂盒（荧光法）可简单、直接地测定多种细胞的葡萄糖摄取能力。该试剂盒使用葡萄糖类似物2-脱氧葡萄糖（2-DG）测量葡萄糖摄取，2-DG在测定中被细胞摄取并被己糖激酶磷酸化为2-DG6P。2-DG6P无法进入后续代谢步骤，因而会在细胞中聚集，直接成比例地反映细胞的葡萄糖摄取水平。在该测定中,2-DG的摄取水平通过偶联酶促反应方法测定,其中，2-DG6P在此过程中被氧化生成NADPH，随后NADPH与探针反应，生成的荧光（λex = 535/λem = 587 nm）产物与细胞的2-DG摄取量成比例。
危險聲明
防範說明
危險分類
Aquatic Chronic 3
儲存類別代碼
10 - Combustible liquids
閃點（°F）
188.6 °F - closed cup
閃點（°C）
87 °C - closed cup
Chapter Twenty-Two Mechanisms and Methods in Glucose Metabolism and Cell Death.
Zhao Y, et al.
Methods in Enzymology, 442, 439-457 (2008)
Yang Zhao et al.
Blood, 131(14), 1587-1599 (2018-02-22)
Monocytes and macrophages play a key role in defending pathogens, removing the dead cells or cell debris, and wound healing. The mammalian target of rapamycin (mTOR) inhibitor rapamycin (RPM) is widely used in clinics to treat patients with organ transplantation
Norio Yamamoto et al.
Current protocols in pharmacology, Chapter 12, Unit 12-Unit 12 (2011-12-08)
Facilitative glucose uptake transport systems are ubiquitous in animal cells and responsible for transporting glucose across the cell surface membrane. Evaluation of glucose uptake is crucial in the study of numerous diseases and metabolic disorders, such as myocardial ischemia, diabetes
Norio Yamamoto et al.
Analytical biochemistry, 404(2), 238-240 (2010-05-25)
Previously, we developed a microplate assay to quantitate 2-deoxyglucose (2DG) and 2-deoxyglucose-6-phosphate in samples for in vitro and in vivo use. In this assay system, four different reaction mixtures were used, and the difference in the reactivity of the two
Norio Yamamoto et al.
Analytical biochemistry, 351(1), 139-145 (2006-01-31)
A nonradioisotope, 96-well-microplate assay to evaluate glucose uptake activity in cultured cells has been developed. 2-Deoxyglucose (2DG) was detected by measuring a potent fluorophore, resorufin, generated after incubation with a single assay solution containing hexokinase, adenosine 5'-triphosphate, glucose 6-phosphate dehydrogenase
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