推荐产品
质量水平
表单
powder
技术
cell culture | mammalian: suitable
组分
phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no
运输
ambient
储存温度
2-8°C
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一般描述
McCoy 5A培养基推荐用于在原代细胞培养中生产病毒。
应用
McCoy 5A培养基已用于培养：
- 人结肠癌细胞系（HCT116、RKO、FET、CBS、HCT116b和TENN），以研究富亮氨酸重复G蛋白偶联受体（LGR5）在结肠癌转移中的作用
- 人结肠HCT116细胞，以研究miR-224在肿瘤中的过表达
- 成骨细胞，以研究缺钙羟基磷灰石在标准细胞培养基中的离子反应性
生化/生理作用
McCoys 5A培养基起初是通过修改BME中的氨基酸，用来支持肝肿瘤细胞。 该配方还被用来支持骨髓、皮肤、牙龈、肾脏、大网膜、肾上腺、肺、脾、大鼠胚胎和其他细胞类型原代培养的生长。
数量
按照每升培养基11.9克粉末配制。
重悬
补充2.2 g/L碳酸氢钠。
补充剂
通常也和此产品一起购买
产品编号
说明
价格
储存分类代码
11 - Combustible Solids
WGK
WGK 3
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Yan Zhang et al.
Oncotarget, 8(53), 91134-91149 (2017-12-07)
Human osteosarcoma is considered a malignant tumor with poor prognosis that readily metastasizes. Tetrahydrocurcumin (THC) has been reported to have anti-tumor activity in numerous tumors. In addition, hypoxia-inducible factor-1α (HIF-1α) has been demonstrated to be associated with tumor metastasis by
Yu Wang et al.
PloS one, 8(7), e68744-e68744 (2013-08-08)
MicroRNA-224 (miR-224) is frequently over-expressed in liver and colorectal cancers. We and others have previously described the role of miR-224 over-expression in cell proliferation in vitro but we have yet to identify the relevant miR-224 direct target. In this study
Ying Liu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 44(4), 1396-1410 (2017-12-05)
Osteosarcoma (OS) is the most common primary malignant tumor of bone tissue. Although treatment effectiveness has improved, the OS survival rate has fluctuated in recent years. Andrographolide (AG) has been reported to have antitumor activity against a variety of tumors.
Archana Kumari et al.
The EMBO journal, 39(14), e104006-e104006 (2020-06-23)
Cellular studies of filamentous actin (F-actin) processes commonly utilize fluorescent versions of toxins, peptides, and proteins that bind actin. While the choice of these markers has been largely based on availability and ease, there is a severe dearth of structural
Xin Zhang et al.
Cellular and molecular gastroenterology and hepatology, 12(4), 1179-1199 (2021-06-05)
Chronic inflammation in colon section is associated with an increased risk of colorectal cancer (CRC). Proinflammatory cytokines were produced in a tumor microenvironment and correlated with poor clinical outcome. Tumor-infiltrating T cells were reported to be greatly involved in the
商品
McCoy's 5A Modified Media Formulation
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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