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M3024

Sigma-Aldrich

Eagle最低必需培养基

with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture

别名:

MEM

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

500

表单

powder

技术

cell culture | mammalian: suitable

组分

Earle’s salts (5% CO2): yes
L-glutamine: no
HEPES: no
NaHCO3: no
sodium pyruvate: no
phenol red: no

运输

ambient

储存温度

2-8°C

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相关类别

一般描述

Eagle最低必需培养基含有19种氨基酸。包括必需氨基酸和非必需氨基酸：丙氨酸(L-ala)、天冬酰胺(L-asn)、天冬氨酸(L-asp)、谷氨酸(L-glu)、甘氨酸(L-gly)、脯氨酸(L-pro)和丝氨酸(L-ser)。可优先用于在减少的血清存在下的贴壁细胞的生长或作为开发无血清培养基的基础配方。适合多种细胞的单层培养。

应用

Eagle最低必需培养基已用于：

  • 培养Ishikawa子宮內膜腺癌细胞，用于双酚研究
  • 培养MCF-7细胞，用于激素处理实验
  • 维持斑马鱼松果体活性，用于作用光谱分析

特点和优势

含有高浓度的氨基酸，因此培养基成分类似于哺乳动物培养细胞的蛋白质成分。

数量

配制成每升培养基含 9.3 克粉末。

重悬

补充 0.292 g/L左旋谷氨酰胺、2.2 g/L 碳酸氢钠。

补充剂

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说明
价格

储存分类代码

13 - Non Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000419267
0000419267
0000380459
0000380454
0000380111

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商品

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

实验方案

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

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