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Merck

L3414

Sigma-Aldrich

Monoclonal Anti-LAP2 (TMPO) antibody produced in mouse

~2 mg/mL, clone 6E10, purified immunoglobulin, buffered aqueous solution

别名:

Anti-CMD1T, Anti-Cardiomyopathy, dilated, 1T, included, Anti-Lamina Associated Polypeptide 2/Thymopoietin, Anti-TMPO, Anti-Thjymopoietin

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

6E10, monoclonal

形狀

buffered aqueous solution

分子量

antigen 25 kDa (ζ isoform)
antigen 38 kDa (γ isoform, singlet or doublet)
antigen 42 kDa (δ isoform)
antigen 45 kDa (ε isoform)
antigen 50 kDa (β isoform, singlet or doublet)
antigen 76 kDa (α isoform)

物種活性

human

濃度

~2 mg/mL

技術

immunocytochemistry: suitable
microarray: suitable
western blot: 2-4 μg/mL using whole extract of cultured human chronic myelogenous leukemia K562 cells.

同型

IgG1

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... TMPO(7112)

一般說明

Monoclonal Anti-LAP2 (TMPO) (mouse IgG1 isotype) is derived from the 6E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide (amino acids 29-50) common to the N-terminus of all TMPOs. Thymopoietin β (TMPOβ)/lamina associated polypeptide 2 (LAP2) is a type II integral protein of the inner nuclear envelope (NE). In mammalian cells, six alternatively spliced TMPO isoforms, designated α, β, β′, γ, δ, ε, and ζ have been isolated and characterized. LAP2/TMPO proteins is highly conserved in mammals and Xenopus. The expression of the various TMPO isoforms is ubiquitous, with higher levels in proliferative tissues. It has four domains: a hydrophilic C-terminus domain, a hydrophobic transmembrane domain, a NE targeting and lamina-binding domain, and a chromatin binding domain. TMPOβ/LAP2 contains two putative p34cdc2 kinase phosphorylation sites.

免疫原

synthetic peptide corresponding to amino acids 29-50 common to the N-terminus of all human TMPOs (isoforms α, β, γ, δ, ε, and ζ).

應用

Monoclonal Anti-LAP2 (TMPO) antibody produced in mouse may be used in immunoblotting and immunocytochemistry.

生化/生理作用

Thymopoietin β (TMPOβ)/lamina associated polypeptide 2 (LAP2) is putatively involved in functional nuclear architecture and cell cycle control. TMPOβ/LAP2 plays a key role in nuclear envelope (NE) disassembly and reassembly during mitosis and linking chromatin to the NE in interphase. TMPOβ/LAP2 binds lamin B1 and chromosomes in a phosphorylation dependent manner. TMPOβ/LAP2, either together with germ-cell-less (GCL) or alone, can repress the transcriptional activity of the elongation factor 2-dimerization partner (E2F-DP) heterodimer. TMPOα/LAP2 has a role in regulating the dynamics of the nuclear lamina and this interaction is required for nuclear growth after mitosis.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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在文件库中查找您最近购买产品的文档。

访问文档库

Review: Lamina-Associated Polypeptide 2 Isoforms and Related Proteins in Cell Cycle-Dependent Nuclear Structure Dynamics
Dechat T, et al.
Journal of Structural Biology, 129(2), 335-345 (2000)
Detergent-salt resistance of LAP2alpha
Dechat T, et al.
The Embo Journal, 17(16), 4887-4902 (1998)

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