GF197

Vaccinia Virus B18R protein, recombinant expressed in HEK 293 cells, Carrier-Free

recombinant, expressed in HEK 293 cells, endotoxin tested, suitable for cell culture, carrier-free

• 别名: B18, Soluble interferon alpha/beta receptor B18
• 分類程式碼代碼: 12352202
• NACRES: NA.55

属性

• 重組細胞: expressed in HEK 293 cells
• 品質等級: 200
• 化驗: ≥98% (SDS-PAGE)
• 形狀: liquid
• 效力: Supports transfection of Simplicon-based TagGFP RNA
• 分子量: 50 kDa (glycosylated)
• 技術: cell culture | mammalian: suitable
• 適合性: endotoxin tested
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: vaccinia virus ... B18R(3707576)

说明

一般說明

Recombinant B18R from vaccinia virus is expressed in human HEK 293 cells as an N-terminally his-tagged glycoprotein with a calculated molecular mass of 38.4 kDa. The DTT-reduced protein migrates as a ~50 kDa polypeptide on SDS-PAGE due to glycosylation. The non-reduced protein migrates as a ~46 kDa polypeptide on SDS-PAGE due to internal cystines. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture.

應用

B18R protein can be used with the Simplicon RNA Reprogramming Technology (Catalog No. SCR550) to create “foot-print” free human iPS cells at the highest efficiency without unwanted RNA degradation caused by cellular IFN responses.

生化／生理作用

B18R is a vaccinia virus gene product that acts as a decoy receptor for Type I Interferons (IFNs). B18R binds type I IFNs more avidly than their receptor. This protein has been shown to inhibit all IFN-α subtypes and IFN-β, but not type II or III IFNs. B18R enables increased cell viability during RNA transfection protocols designed to convert human somatic donor cells into iPSCs via direct delivery of synthetic mRNA′s. As a type I IFN receptor, B18R has a broad species specificity. It has significant regions of homology with the alpha subunits of the mouse, human, and bovine type I IFN receptors. Binding of soluble recombinant B18R to cells protected the cultures from IFN and allowed vaccinia virus replication. B18R can be used with the Simplicon RNA Reprogramming Technology (Cat. No. SCR550) to create “foot-print” free human iPS cells at the highest efficiency without unwanted RNA degradation caused by cellular IFN responses.

外觀

This product is supplied as a 50 μg liquid solution, with 10 mM phosphate, 0.5 M NaCl, pH 7.5 buffer. It is aseptically filled. The protein concentration is 300-700 μg/ml (by the BCA method). For exact concentration see the product′s Certificate of Analysis.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable