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Key Documents

GEE70073X

Exonuclease I, 10 U/μL

Cytiva E70073X, pack of 5000 units

别名:

Exonuclease I Enzyme

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.53

生物源

Escherichia coli

包裝

pack of 5000 units

製造商／商標名

Cytiva E70073X

運輸包裝

dry ice

儲存溫度

−20°C

相关类别

一般說明

Acts specifically on single-stranded DNA degrading it processively in the 3′- to 5′-direction, producing 5′-mononucleotides.
Exonuclease I (Exo I) from E. coli is an Mg2+ ion dependent exonuclease.

應用

Exonuclease I has been used to quantify the expression of 56 genes in each F2 XY gonad.

生化／生理作用

Exonuclease I (Exo I) from E. coli can degrade single-stranded DNA (ssDNA) from 3′ to 5′. It participates in various DNA repair systems, like, RecBCD-dependent homologous recombination, methyl-dependent mismatch repair and genomic tandem repeat maintenance.

特點和優勢

  • Elimination of residual single-stranded DNA containing a 3′-terminus.
  • Measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA.
  • Measuring DNA helicase activity.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

儲存類別代碼

12 - Non Combustible Liquids

分析证书(COA)

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Structural and Biochemical Studies of a Moderately Thermophilic Exonuclease I from Methylocaldum szegediense
Fei L, et al.
Testing, 10(2), e0117470-e0117470 (2015)
Elucidation of the transcription network governing mammalian sex determination by exploiting strain-specific susceptibility to sex reversal
Munger S C, et al.
Genes & Development, 23(21), 2521-2536 (2009)

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