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GE29-0181-83

HiPrep SP High Performance16/10

Cytiva 29-0181-83, pack of 1 column

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About This Item

UNSPSC代码:
13111000
NACRES:
NA.56

ligand

sulphopropyl

描述

Ion Exchanger Type (value)

包装

pack of 1 column

制造商/商品名称

Cytiva 29-0181-83

参数

1-5 ml/min flow rate
44 psi

柱床尺寸

16 mm × 100 mm

色谱柱内径

16 mm

基质

6% cross-linked agarose

粒径

24-44 μm

平均零件尺寸

34 μm

cleaning

3-14

工作范围

4-13

适用性

suitable for bioprocess medium

一般描述

HiPrep SP HP 16/10 is a strong cation exchanger designed for preparative ion exchange chromatography separations. This 20 mL column is prepacked with SP Sepharose High Performance chromatography resin. The resin is based on rigid, highly cross-linked beaded agarose with a mean bead diameter of 34 µm for high resolution separations. High mechanical strength of the matrix allows for high flow rates, together with outstanding resolution. Capacities vary according to the protein applied and its concentration. Loading capacity will also vary depending on the salt concentration, pH, and temperature at which the protein is applied.

特点和优势

  • For high-performance preparative scale separations where high efficiency and high resolution are desired.
  • Highly cross-linked agarose provides excellent chemical and physical stability.
  • Slim, modern design allows for easy handling and connection.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

HiPrep is a trademark of Cytiva

象形图

Flame

警示用语:

Warning

危险声明

储存分类代码

3 - Flammable liquids


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

实验方案

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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