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Merck
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Key Documents

E8022

Sigma-Aldrich

pRc/RSV Expression vector

set of expression vectors powered by either the CMV2 or the RSV promoter

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About This Item

MDL號碼:
MFCD01094969
分類程式碼代碼:
41106617
NACRES:
NA.85

等級

for molecular biology

形狀

buffered aqueous solution

儲存溫度

−20°C

相关类别

一般說明

pRc and pRSV Supercoiled Expression Vectors (5.2 kb) are for protein expression in mammalian cells. Each vector contains:
  • The polyadenylation signal and transcription termination sequence from BGH to enhance mRNA stability
  • The SV40 replication origin for episomal replication
  • The f1 origin for rescue of the sense strand
  • The neomycin resistance gene, and
  • An ampicillin resistance gene and colE1 origin for selection and maintenance in E. coli.


The primary difference between these expression vectors is that pRc contains the enhancer-promoter sequence from human cytomegalovirus (CMV2) and pRSV from Rous sarcoma virus (RSV). Both are strong promoters for high-level transcription.

The pRc MCS contains unique ApaI, HindIII, NotI, and XbaI restriction sites, plus redundant BstXI sites.

The pRSV MCS contains unique HindIII, NotI, SpeI, and XbaI restriction sites, plus redundant BstXI sites.

特異性

The MCS contains unique ApaI (CMV2 only), HindIII, NotI, SpeI (RSV only), and XbaI restriction sites, plus redundant BstXI sites.

特點和優勢

Supercoiled vectors for protein expression in mammalian cells. Both of these vectors contain:
  • the polyadenylation signal and transcription termination sequence from BGH to enhance mRNA stability
  • the SV40 replication origin for episomal replication
  • the f1 origin for rescue of the sense strand
  • the neomycin resistance gene, and
  • an ampicillin resistance gene and colE1 origin for selection and maintenance in E. coli.
The primary difference between these expression vectors is that one contains the enhancer-promoter sequence from human cytomegalovirus (CMV2) and the other from Rous sarcoma virus (RSV). Both are strong promoters for high-level transcription.

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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