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Key Documents

D8037

Sigma-Aldrich

Driselase 崩溃酶, 来自担子菌属

BioReagent, suitable for plant cell culture

别名:

Driselase崩溃酶 来源于担子菌

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
10171502
NACRES:
NA.72

產品線

BioReagent

品質等級

形狀

powder

成份

Protein, ≥10% biuret

技術

cell culture | plant: suitable

應用

agriculture

儲存溫度

−20°C

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應用

崩溃酶 担子菌属已用于:

  • Coccomyxa细胞的球质体制备
  • 基于CRISPR/Cas9的诱变方案,用于短柄草及其异源多倍体亲族Brachypodium hybridum
  • 用于细胞壁消化,以对莲藕根组织进行全标本包埋免疫定位

生化/生理作用

崩溃酶具有天然混合酶活性(真菌碳水化合物酶),可用于消化植物细胞壁以促进植物材料的浸渍、原生质体形成和提取过程。崩溃酶可释放细胞壁内的碳水化合物。本配方包含纤维素、内切-1,3-β-葡聚糖酶和木聚糖酶的酶活性。

其他說明

粗体粉末中含有昆布多糖酶、木聚糖酶和纤维素酶。

法律資訊

Driselase is a trademark of ASKA Animal Health Co. Ltd.

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Dieter Hackenberg et al.
Plant physiology, 172(2), 1154-1166 (2016-08-24)
In this study, we report the functional characterization of heterotrimeric G-proteins from a nonvascular plant, the moss Physcomitrella patens. In plants, G-proteins have been characterized from only a few angiosperms to date, where their involvement has been shown during regulation
M C Ralet et al.
Carbohydrate research, 263(2), 227-241 (1994-10-17)
Cell walls from sugar-beet pulp contain some feruloyl groups linked to the pectic neutral side-chains. Enzymic as well as chemical hydrolysis of the pulp yielded a series of feruloylated oligosaccharides, which have been purified by Sephadex LH-20 and Biogel P-2
D T Kaplan et al.
Journal of nematology, 22(3), 399-406 (1990-07-01)
Radopholus spp. were reared in carrot tissue culture via established procedures, with slight modification. Several plant tissue maceration enzymes and flotation media (salts and sucrose) were evaluated with regard to nematode toxicity and extraction efficiency. Best extraction of viable nematodes
T Ishii et al.
Carbohydrate research, 248, 179-190 (1993-10-04)
Hydrolysis of spinach-leaf cell walls with Driselase (a fungal enzyme preparation) released two arabino-oligosaccharides and one galactobiose, each carrying a ferulic acid moiety. The oligosaccharides were characterized by NMR spectroscopy, methylation analysis, and FABMS. They were O-(2-O-trans-feruloyl-alpha-L-arabinofuranosyl)-(1-->5)-L-arabinof uranose, O-(6-O-trans-feruloyl-beta-D-galactopyranosyl)-(1-->4)-D-galactopy ranose
D P Blowers et al.
Plant physiology, 86(2), 505-509 (1988-02-01)
Plasma membrane vesicles from wild carrot cells grown in suspension culture were isolated by aqueous two-phase partitioning, and ATP-dependent phosphorylation was measured with [gamma-(32)P]ATP in the presence and absence of calcium. Treatment of the carrot cells with the cell wall

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