8505C

94090184, human thyroid, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: 8505C, 94090184
• 生物源: human thyroid
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human thyroid carcinoma, undifferentiated.

細胞系描述

Established from undifferentiated thyroid carcinomas of a 78 year old female patient. Pathologically this primary carcinoma tissue contained residual well differentiated components suggesting well differentiated to undifferentiated carcinoma progression. Tumour suppresser genes p53, Rb, APC and MCC were analysed and sequence analysis confirmed a C:G to G:C transversion at the first base of p53 gene codon 248. Polymerase chain reaction-loss of heterozygosity assays confirmed allelic deletion of p53 gene. Loss of heterozygosity of tumour suppresser genes was not observed.

應用

8505C cell line has been used to study the impact of synthetic manipulation of expression of microRNAs miR-25 and miR-222 in benign and malignant thyroid cells. It has also been used to determine the induction of pluripotency markers by mitogen activated protein kinase (MEK) inhibition.

培養基

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Doubling time is 36 hours. Saturation density at confluency is 1x100,000 cells/cm².

其他說明

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