HIN, JP human cell line

IHW Number: 9163, 94082263

• UNSPSC代码: 41106514

属性

• 生物来源: human blood
• 种族: Caucasian
• 性别: not specified
• 生长模式: Suspension
• 核型: Not specified
• 形态学: Lymphoblastoid
• 技术: cell culture | mammalian: suitable
• 运输: dry ice

说明

一般描述

Consanguineous: No

Homozygous: No

细胞系描述

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA图谱分析

HLA DRB1: 0301 0803
HLA DRB3: 101
HLA DQAP: 4.1 4.2
HLA DQA1: 0601 0501
HLA DQB1: 0301 0201
HLA DPB1: 0301 0401

血清学分析

HLA A: 1 2
HLA A ief: 2.2
HLA B: 8 51
HLA B ief: 51.1

培养基

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

传代培养常规

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.