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Key Documents

JEG3

92120308, human placenta, Epithelial

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About This Item

分類程式碼代碼:
41106514

product name

JEG3, 92120308

生物源

human placenta

增長模式

Adherent

染色體組型

modal no. 71

形態學

Epithelial

產品

Human chorionic gonadotrophin (hCG), somatomammotrophin

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Human choriocarcinoma

細胞系描述

One of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. Cell line releases chorionic gonadotrophin and somatomammotrophin and progesterone. It is also able to transform steroid precursors to oestrone and oestradiol. JEG3 has the same DNA profile as BeWo (Sigma Catalogue number 86082803); it was established by serial cloning of BeWo .

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin, 5% CO2; 37°C. Vacuolisation will occur at confluency.

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