CCD 18Lu

90112706, human lung, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: CCD 18Lu, 90112706
• 生物源: human lung
• 增長模式: Adherent
• 染色體組型: 2n = 46
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human lung

細胞系描述

Established from the lung tissue of a 2.5 month old female with cerebral anoxia, cardiac anomaly, sepsis, endocardial cushion defect and foetal alcoholic syndrome. The cells can undergo up to 19 population doublings. CCD 18Lu (90112706) and CCD-18Co (90070503) were derived from the lung and colon respectively from the same patient; this has been confirmed by STR profiling at ECACC. Ethnicity: Black.

應用

Ageing and genetic studies

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Following resuscitation this cell line is initially slow to start.

其他說明

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