MRC-5 SV1 TG1

85042501, human lung (fetal), Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: MRC-5 SV1 TG1, 85042501
• 生物源: human lung (fetal)
• 增長模式: Adherent
• 染色體組型: Modal no. = 60-80 hyperdiploid
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human foetal lung, SV40 transformed

細胞系描述

A clonal derivative of an SV40 transformed permanent line of MRC-5. Selection was made using X-ray induction in the presence of 2μg/ml thioguanine. The cells are HAT sensitive.

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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