AB-1 HA

12070506, mouse mesothelium, epithelial; small, spindly morphology, cobblestone at confluence

• 别名: AB 1 HA, AB-1HA
• UNSPSC代码: 41106514

属性

• 产品名称: AB-1 HA, 12070506
• 生物来源: mouse mesothelium
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Epithelial; cells are small and can be spindly at low density but form a uniform cobblestone epithelial layer at near confluence
• 受体: Not specified
• 相关疾病: cancer

说明

细胞系来源

Mouse mesothelioma cell line transfected with influenza haemagglutinin gene (HA)

细胞系描述

AB-1 HA is a mouse mesothelioma cell line transfected with influenza haemagglutinin gene (HA). Mice (BALB/c strain, female, 6-8 weeks old) were exposed to crocidolite asbestos through intraperitoneal injection, resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. The resulting celll ine, designated AB-1 was transfected with influenza haemagglutinin gene (HA) in order to generate an endogenous tumour antigen. Cell Bank Australia reference number: CBA1374.

DNA图谱分析

Not specified

培养基

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + FBS / FCS (F2442) + 400ug/ml G418.

传代培养常规

Optimal split ratio 1:10 - 1:20 (seeding density 0.7 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. Cells will require passaging twice weekly at this seeding density.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Richard Lake - University of Western Australia, Australia