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生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
CB59-4B11, monoclonal
形狀
buffered aqueous solution
分子量
antigen ~25 kDa
物種活性
rat, human
濃度
~1.5 mg/mL
技術
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 4-8 μg/mL using HCT-116 cells.
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... CTSB(1508)
rat ... CTSB(64529)
一般說明
Monoclonal Anti-Cathepsin B specifically recognizes human1 and rat1 cathepsin B (~ 25 kDa). The antibody epitope resides within the peptide sequence EPGYSP which corresponds to amino acids 212-217 of human cathepsin B. The antibody does not cross react with the closely related lysosomal cysteine proteases: cathepsins L, H, K, S, V, and W
The CTSB (Cathepsin B) gene encoding cathepsin B is mapped to human chromosome 8p23.1. Cathepsin B are normally localized in lysosomes of almost all mammalian cells, but under certain conditions they can be secreted from the cell that take part in local proteolysis. Cathepsin B is both an endo- and exopeptidase, composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor.
免疫原
Recombinant human cathepsin B.
應用
indirect ELISA, immunohistochemistry, In HCT-116 cells. 4-8μg/mL of antibody concentration may be used. A dilution of 1:800 in human pancreatic carcinoma cell line PANC-1 was used for immunoblotting
生化/生理作用
Cathepsins are lysosomal proteases activation of enzyme precursors, and tumor invasion and metastasis. It is involved in the proteolysis of many proteins involved in different physiological and pathological pathways. In the brain, this protease, also known as amyloid precursor protein secretase, is responsible for the proteolytic processing of amyloid precursor protein (APP). An incomplete proteolytic processing of APP has been found as a causative factor in Alzheimer disease. Furthermore, ablated genes coding cathepsin B and L leads to enormous neuronal cell death,probably due to autophagy abnormalities. Cathepsin B has been implicated in tumor progression by virtue of localization at the invading front of the tumor. For example, blocking cathepsin B expression in human glioblastoma SNB19 cells by a stable transfection of an expression vector expressing antisense cathepsin B, caused suppression of glioblastoma-induced neovascularization.
Monoclonal Anti-Cathepsin B specifically recognizes human1 and rat1 cathepsin B (~ 25 kDa). The antibody epitope resides within the peptide sequence EPGYSP which corresponds to amino acids 212-217 of human cathepsin B. The antibody does not cross react with the closely related lysosomal cysteine proteases: cathepsins L, H, K, S, V, and W.
Cathepsins significantly takes part in the immune response, by processing antigens into peptides for its presentation through MHC class II molecules.
Monoclonal Anti-Cathepsin B specifically recognizes human1 and rat1 cathepsin B (~ 25 kDa). The antibody epitope resides within the peptide sequence EPGYSP which corresponds to amino acids 212-217 of human cathepsin B. The antibody does not cross react with the closely related lysosomal cysteine proteases: cathepsins L, H, K, S, V, and W.
Cathepsins significantly takes part in the immune response, by processing antigens into peptides for its presentation through MHC class II molecules.
外觀
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
Cysteine Cathepsins and the Cutting Edge of
Cancer Invasion
Cancer Invasion
Cell Cycle, 6:1, 60-64 (2007)
Protease signalling in cell death: caspases versus cysteine cathepsins
Febs Letters, 581, 2761?2767-2761?2767 (2007)
Enzymatically active lysosomal proteases are associated with
amyloid deposits in Alzheimer brain
amyloid deposits in Alzheimer brain
Proceedings of the National Academy of Sciences of the USA, 87, 3861-3865 (1990)
Localization and Activity of Various Lysosomal Proteases in
Leishmania amazonensis-Infected Macrophages
Leishmania amazonensis-Infected Macrophages
Infection and Immunity, 58, 1730-1737 (1990)
Blockade of cathepsin B expression in human glioblastoma cells is
associated with suppression of angiogenesis
associated with suppression of angiogenesis
Oncogene, 23, 2224?2230-2224?2230 (2004)
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