C-39221

Endothelial Cell Growth Medium MV 2

SupplementPack containing all media supplements as individual vials, 1 Pack for 500 ml

• 别名: Primary Cell Culture Media, Recommended with C-22221
• 分類程式碼代碼: 12352207
• NACRES: NA.71

属性

• 形狀: liquid
• 包裝: pkg of 1 kit
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −20°C

说明

一般說明

Endothelial Cell Growth Medium MV 2 SupplementPack (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-22221

應用

Endothelial Cell Growth Medium MV2 was developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta. The media are optimized for primary human cells but can also be used for bovine, porcine, murine, rat and dog endothelial cells from microvascular vessels as well as for various endothelial cell lines.Recommended for:Human Dermal Microvascular Endothelial Cells (HDMEC)Human Coronary Artery Endothelial Cells (HCAEC)Human Aortic Endothelial Cells (HAoEC)Human Pulmonary Microvascular Endothelial Cells (HPMEC)Human Dermal Lymphatic Endothelial Cells (HDLEC)Human Dermal Blood Endothelial Cells (HDBEC)Human Cardiac Microvascular Endothelial Cells (HCMEC)Human Uterine Microvascular Endothelial Cells (HUtMEC)Note: For best transfection results we recommend using PromoCell heparin-free Endothelial Cell Growth Medium MV2. Heparin, included all our other our Endothelial Cell Growth Media formulations, may reduce transfection efficiency.

成分

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

品質

All lots of PromoCell Endothelial Cell Media MV/MV2 are subjected to comprehensive quality control tests using primary human endothelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested endothelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

準備報告

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlEpidermal Growth Factor (recombinant human): 5 ng / mlBasic Fibroblast Growth Factor (recombinant human): 10 ng / ml Insulin-like Growth Factor (Long R3 IGF): 20 ng / ml Vascular Endothelial Growth Factor 165 (recombinant human): 0.5 ng / mlAscorbic Acid: 1 µg / mlHydrocortisone: 0.2 µg / ml

儲存和穩定性

Store the SupplementPack at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.Note: The SupplementPack is delivered thawed and can be frozen after arrival without losing any activity.

例行更新培養

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安全信息

• 象形圖: GHS02,GHS07
• 訊號詞: Danger
• 危險聲明: H225,H319
• 防範說明: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
• 危險分類: Eye Irrit. 2 - Flam. Liq. 2
• 儲存類別代碼: 3 - Flammable liquids
• 閃點（°F）: 55.4 °F
• 閃點（°C）: 13 °C