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生物源
bovine liver
形狀
aqueous suspension
比活性
10,000-40,000 units/mg protein
分子量
tetramer ~250 kDa
濃度
20-50 mg/mL
技術
GC/MS: suitable
等電點
5.4
UniProt登錄號
運輸包裝
wet ice
儲存溫度
2-8°C
InChI
1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
InChI 密鑰
NUVBSKCKDOMJSU-UHFFFAOYSA-N
基因資訊
cow ... CAT(280743)
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一般說明
来自牛肝的过氧化氢酶包含506种残基。它是一种四聚体，每个单体对应的分子量为61 kDa。每个单体的活性位点包括烟酰胺腺嘌呤二核苷酸磷酸(NADPH)和铁结合区。
研究领域：细胞信号传导
研究领域：细胞信号传导
Research Area: Cell Signaling
Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.
Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.
應用
牛肝中的过氧化氢酶用于以下应用：
- 介电泳场流分级法（DEP-FFF）：这是一种色谱方法，其中细胞洗脱时间分别在沉降、DEP和流体动力升力（FSED、FDEP和FHDL）的控制下反映细胞在流体动力流剖面中的位置。DEP-FFF缓冲液由过氧化氢酶和其他成分组成。
- 气相色谱-质谱分析。
- 去离子。
- 气相色谱-火焰离子化检测器分析
- 血管功能分析。
牛肝中的过氧化氢酶用于测量癌组织匀浆中的过氧化氢含量。它还被用于测试有机磷杀虫剂毒死蜱-乙基(CE) [0,0-二乙基 0 (3,5,6-三氯-2-吡啶基) 硫代磷酸]对其酶活性的影响。
过氧化氢酶作为天然抗氧化剂，可用于研究活性氧物质对基因表达和凋亡的作用。它还被用于保护蛋白质、脂质和核酸免受氧化损伤。 工业上，过氧化氢酶已被用于去除添加至牛奶和奶酪中以及纺织品漂白使用的过氧化氢，并用于检查其对DNA修复大肠杆菌突变体活力的阳性效应。
生化／生理作用
本品不需要任何活化剂，但其活性可被3-氨基-1-H-1,2,4三唑、氰化物、叠氮化物、羟胺、溴化氰、2-巯基乙醇、二硫苏糖醇、邻联茴香胺和硝酸盐抑制。
Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.
注意
催化剂的溶液不应冻结。冷冻溶液会导致50-70%的活性损失。
單位定義
一单位酶可在pH7.0、25℃下每分钟分解1.0 μM的H2O2，通过测量A240的下降速率可知H2O2浓度从10.3降至9.2 mM。
準備報告
该产品为含0.1%百里酚的结晶悬浮液，活性为10，000-40，000单位/毫克。
儲存和穩定性
Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
persons
抑制劑
产品编号
说明
价格
相關產品
产品编号
说明
价格
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US), type N95 (US)
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