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Merck

C-12921

Sigma-Aldrich

Human CD34+ Progenitor Cells (hCD34+-CB)

Isolated from cord blood, single donor, 100,000 cryopreserved cells

别名:

hCD34+-CB cells

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About This Item

分類程式碼代碼:
41106514
NACRES:
NA.81

生物源

human umbilical cord blood

包裝

pkg of 100,000 cells

形態學

(progenitor)

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−196°C

一般說明

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

細胞系來源

Cord Blood

應用

CD34 is a glycosylated transmembrane protein and represents a well-known marker for primitive blood- and bone marrow-derived progenitor cells, especially for hematopoietic and endothelial stem cells. Although the biological functions of CD34 are largely unknown, recent data suggest that CD34 is involved in maintenance of the progenitor cells in a phenotypically undifferentiated state. PromoCell offers CD34+ Progenitor Cells from the cord blood of healthy donors. The CD34+ Progenitor Cells contain two main cellular subpopulations, hematopoietic and endothelial progenitor cells. Therefore, CD34+ Progenitor Cells are suitable for a series of studies, e.g. directed differentiation into more committed types of blood cells and endothelial lineages.

品質

Rigid quality control tests are performed for each lot of CD34+ Progenitor Cells. They are routinely characterized by flow cytometry analyzing the viability, cell size, and the presence of significant markers, i.e. CD34. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

警告

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

例行更新培養

Click here for more information.

其他說明

Recommended Plating Density: 5000 cells per mlTested Markers: CD34 positive

推薦產品

Recommended Primary Cell Culture Media:Link

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Tatjana Weidner et al.
Nature protocols, 16(7), 3210-3240 (2021-04-14)
Receptor targeting of vector particles is a key technology to enable cell type-specific in vivo gene delivery. For example, T cells in humanized mouse models can be modified by lentiviral vectors (LVs) targeted to human T-cell markers to enable them

商品

CD34+ Progenitor Cells are suitable for diverse studies, as well as for the directed differentiation into more committed blood cell phenotypes and endothelial lineages. 

什么是原代细胞?关于动物和人类原代细胞系和培养基的全面概述,以及它们如何应用于生物医学细胞培养应用。相对于细胞系,原代培养物的均一性较低。

Yeast plasmid selection: Utilizes auxotrophic yeast strains for plasmid selection, enabling growth on synthetic media for better yield.

Yeast plasmid selection: Utilizes auxotrophic yeast strains for plasmid selection, enabling growth on synthetic media for better yield.

实验方案

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

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