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重組細胞
expressed in E. coli (C-terminal histidine tagged)
品質等級
化驗
≥90% (SDS-PAGE)
形狀
buffered aqueous solution
比活性
≥1,000 units/mg protein
分子量
~30 kDa
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... CASP8(841)
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一般說明
Human recombinant C−terminal histidine tagged caspase 8 is expressed as the truncated pro-caspase 8 (amino acids 213−496). It is a fully active protein consisting of 18 kDa and 12.4 kDa subunits; the 12.4 kDa subunit contains the histidine tag.
生化／生理作用
Caspase 8 is at the top of the hierarchy of the caspase cascade and is considered an initiator caspase. It exists in the cell as an inactive 55 kDa proenzyme. It is converted to the active form, consisting of 18 kDa and 11 kDa subunits, upon its recruitment to the cytoplasmic domain of activated death receptors such as CD95 or TNFR via the adapter protein FADD. The activation of the proenzyme is triggered by the protein′s aggregation, which leads to auto- or transprocessing. Caspase 8 activates downstream caspases (caspases 3, 6, and 7) that cleave key cellular substrates and lead to apoptotic death of the cells.
單位定義
One unit will cleave 1.0 nmol of Ac-Ile-Glu-Thr-Asp-pNA per min at pH 7.4 at 25 °C.
外觀
Solution in 10% sucrose containing 20 mM Tris-HCl, 150 mM imidazole-HCl, pH 8.0, 20 mM 2-mercaptoethanol, 500 mM NaCl, 2.5 mM EDTA, and 0.1% CHAPS.
訊號詞
Danger
危險分類
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - Skin Sens. 1
儲存類別代碼
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
A Prakasam et al.
Cell death & disease, 5, e1358-e1358 (2014-08-01)
BH3 interacting-domain death agonist (Bid) is a BH3-only pro-apoptotic member of the Bcl-2 family of proteins. Its function in apoptosis is associated with the proteolytic cleavage to the truncated form tBid, mainly by caspase-8. tBid translocates to mitochondria and assists
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