推荐产品
生物来源
mouse
质量水平
重组
expressed in mouse cell line
偶联物
alkaline phosphatase conjugate
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
FL-D6, monoclonal
表单
buffered aqueous solution
技术
dot blot: 1:80,000 using human IgG/FITC monoclonal anti-human IgG
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using FITC conjugate
indirect ELISA: 1:30,000
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
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一般描述
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well defined hapten. Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
特异性
该抗体可与游离的和结合的异硫氰酸荧光素(FITC)反应。
免疫原
FITC-BSA偶联物
应用
Alkaline phosphatase-conjugated mouse anti-FITC antibody was used for immunohistochemistry on mouse spleens. Tissue sections were fixed in acetone, blocked with PBS/5% goat serum/ 0.1% Tween and incubated with a FITC-conjugated primary antibody prior to the addition of the AP-conjugated anti-FITC antibody.
Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent such as alkaline phosphatase or horseradish peroxidase. Mouse monoclonal clone FL-D6 anti-FITC−Alkaline Phosphatase conjugate antibody may be used for the detection of FITC and as a universal indicator reagent for bridging FITC with other immunochemical reagents. It can be used in ELISA and immunofluorescent techniques.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
外形
Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 50% glycerol, 1 mM MgCl2 and 15 mM sodium azide
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Martyn A Sharpe et al.
EBioMedicine, 2(9), 1122-1132 (2015-10-27)
The last major advance in the treatment of glioblastoma multiforme (GBM) was the introduction of temozolomide in 1999. Treatment with temozolomide following surgical debulking extends survival rate compared to radiotherapy and debulking alone. However, virtually all glioblastoma patients experience disease
Helder I Nakaya et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(7), 1853-1858 (2016-01-13)
The dynamics and molecular mechanisms underlying vaccine immunity in early childhood remain poorly understood. Here we applied systems approaches to investigate the innate and adaptive responses to trivalent inactivated influenza vaccine (TIV) and MF59-adjuvanted TIV (ATIV) in 90 14- to
H Noorchashm et al.
Journal of immunology (Baltimore, Md. : 1950), 163(2), 743-750 (1999-07-08)
B cell-deficient nonobese diabetic (NOD) mice are protected from the development of spontaneous autoimmune diabetes, suggesting a requisite role for Ag presentation by B lymphocytes for the activation of a diabetogenic T cell repertoire. This study specifically examines the importance
Imadeldin Yahya et al.
Histochemistry and cell biology, 155(1), 145-155 (2020-10-11)
Cell migration plays a crucial role in early embryonic development. The chemokine receptor CXCR4 has been reported to guide migration of neural crest cells (NCCs) to form the dorsal root ganglia (DRG) and sympathetic ganglia (SG). CXCR4 also plays an
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