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Merck
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940822104

CHI 034 human cell line

IHW Number: 9200

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About This Item

分類程式碼代碼:
12352207

生物源

human blood

品質等級

形狀

liquid

種族

Thai

增長模式

Suspension

染色體組型

Not specified

形態學

Lymphoblastoid

運輸包裝

dry ice

一般說明

Consanguineous: No
Homozygous: No

細胞系描述

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA分析

HLA DRB1: 1001 11
HLA DRB3: 202
HLA DQAP: 1.3.4.1
HLA DQA1: 0501 0104
HLA DQB1: 0501 0301
HLA DPB1: 02012 3201

血清學資料

HLA A: 11 19
HLA B: 7 40

培養基

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

例行更新培養

Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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