78363

Atto 490LS maleimide

suitable for fluorescence

• 分類程式碼代碼: 12352111
• NACRES: NA.32

属性

• 化驗: ≥90% (HPLC)
• 形狀: (Report result)
• 分子量: M_w 818 g/mol
• 製造商／商標名: ATTO-TEC GmbH
• 透射率: 254 nm, >90%; 490 nm, >90%
• 螢光: λ_ex 496 nm; λ_em 640.0 nm±10 nm in PBS, pH 7.4
• &lambda ；: in PBS, pH 7.4
• 紫外吸收: λ: 494-500 nm A_max
• 適合性: suitable for fluorescence
• 儲存溫度: −20°C

说明

一般說明

Atto 490LS is a new fluorescent label featuring an extraordinary large Stokes-Shift of 165 nm. Thus the emission spectrum is almost completely separated from its absorption spectrum, making the dye highly suitable for multiplexing experiments, in particular in combination with Atto 488 and Atto 514. Atto 490LS is very hydrophillic and shows excellent water solubility. The dye exhibits a relatively high fluorescence quantum yield, which is only slightly reduced after conjugation to biomolecules, e.g. proteins, even at high degrees of labeling (DOL). Atto 490LS is an anionic dye. After conjugation to a substrate the dye carries a net electrical charge of ^-1.
Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labelling of amines, which usually requires a higher pH than reaction of maleimides with thiols.

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法律資訊

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable