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一般說明
SYBR® Green I是一种特制的不对称花菁染料，用于检测核酸。这种成分由N-烷基化苯并噻唑或苯并噁唑环系统组成，通过单次甲基桥连接吡啶鎓或喹啉鎓环系统。SYBR Green I与dsDNA的小沟结合，激发波长480 nm。其荧光发射峰波长为520nm。
應用
SYBR® Green I核酸凝胶染色剂已用于：
- dsDNA定量
- 聚合酶链式反应（PCR），用于DNA染色
- Comet测定技术
- 评估精子膜的完整性
- 扩增DNA 目视检查（环介导等温扩增（LAMP）法）
- 用作流式细胞术中的荧光染料
- 实时定量逆转录聚合酶链式反应（qRT-PCR），用于逆转录cDNA染色
特點和優勢
- 用于在电泳后对琼脂糖或聚丙烯酰胺凝胶中的双链DNA染色的超灵敏染色剂
- 它还可以检测变性琼脂糖/甲醛和聚丙烯酰胺/尿素凝胶中的ssDNA和RNA，无需任何预洗涤步骤
- 在 Ames 试验中，它的致突变性比溴化乙锭小
- 它对寡核苷酸的检测灵敏度是溴化乙锭的 50-100 倍
- 适用于 DNA 数量有限的许多应用
- SYBR® Green I 与 DNA 的结合不会抑制许多常见限制性内切酶的活性，包括 Hind III 和 EcoR I
- 这种染色剂无需通过凝胶酶解和连接技术去除
- SYBR Green I 是溴化乙锭染色的绿色替代产品
儲存和穩定性
将产品储存在–20 °C。稀释后的染色溶液避光可在2–8 °C保存数周或室温保存3-4天。
法律資訊
SYBR is a registered trademark of Life Technologies
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
201.2 °F - closed cup
閃點（°C）
94 °C - closed cup
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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实验方案
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.
面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。
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