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无菌性
sterile; 0.2 μm filtered
质量水平
表单
solution
包装
pkg of 100 mL (100x)
制造商/商品名称
Roche
浓度
100 ×
技术
cell culture | hybridoma: suitable
杂质
mycoplasma, tested
≤100 EU/mL Endotoxin (LAL test)
溶解性
water: miscible
储存温度
20-25°C
一般描述
Nutridoma is a family of chemically defined supplements that can completely replace serum in cell culture media. Nutridoma-SP is used for the cultivation of established myeloma and hybridoma cells. It is used for the serum-free cultivation of murine myelomas and hybridomas that have an intact cholesterol biosynthesis pathway, such as those derived from the SP2/0, and some of the P3x63Ag8.653 cell lines and lymphoblastoid cell lines, as well as for primary lymphoid cell cultures. It is also used for the serum-free culture of a variety of other cell types, including neural explants.
应用
Nutridoma-SP has been used in the culture media for canine trophoblasts, human embryonic stem cells and PLB-985 cells.
单位定义
Endotoxin (LAL): 10 EU/ml; mycoplasma-tested
外形
Solution, 100x concentrated, filtered through 0.2 μm pore size membrane
Biochemically defined serum-free medium supplement composed of albumin, insulin, transferrin, and other defined organic and inorganic compounds. Nutridoma contains no other growth factors, mitogens, hormones, or sterols.
The solution contains the pH indicator phenol red and appears clear.
Biochemically defined serum-free medium supplement composed of albumin, insulin, transferrin, and other defined organic and inorganic compounds. Nutridoma contains no other growth factors, mitogens, hormones, or sterols.
The solution contains the pH indicator phenol red and appears clear.
制备说明
Working concentration: Nutridoma-SP (100x) is diluted 1:100 (v/v) with sterile basal medium. Use high glucose DMEM/RPMI 1640 (1:1) (R/D medium) for Nutridoma-SP. The final medium should also contain L-glutamine and sodium bicarbonate. The protein concentration is less than 50 μg/ml for 1% working concentration.
Storage conditions (working solution): 2 to 8 °C
Nutridoma working solutions, e.g., media containing 1% Nutridoma (1%) are stable for 4 weeks when stored at 2 to 8 °C in the dark. Do not freeze.
Note: Do not store in plastic as adsorption of ingredients to the plastic surface may occur.
Storage conditions (working solution): 2 to 8 °C
Nutridoma working solutions, e.g., media containing 1% Nutridoma (1%) are stable for 4 weeks when stored at 2 to 8 °C in the dark. Do not freeze.
Note: Do not store in plastic as adsorption of ingredients to the plastic surface may occur.
其他说明
仅用于生命科学研究。不可用于诊断。
法律信息
Nutridoma is a trademark of Roche
储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
No data available
闪点(°C)
No data available
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