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Merck
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Key Documents

SCC050

Sigma-Aldrich

PluriStem C57BL/6N Murine ES Cells

This Murine Embryonic Stem Cell line is derived from C57BL/6N blastocysts.

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About This Item

分類程式碼代碼:
41106509
eCl@ss:
32011203
NACRES:
NA.81

生物源

mouse

品質等級

製造商/商標名

PluriSTEM®

技術

cell culture | stem cell: suitable

輸入

sample type: mouse embryonic stem cell(s)

運輸包裝

liquid nitrogen

一般說明

Embryonic Stem Cell line derived from C57BL/6N blastocysts. C57BL/6N is the most commonly used inbred mouse strain. Large scale mouse knockout projects such as NIH’s KOMP project use C57BL/6 as the standard background for mouse genome engineering. C57BL/6 is also the reference strain for mouse genome sequencing at the NHGRI. C57BL/6N mice show good breeding performance. As a commonly used reference strain, transgenic mouse models are often backcrossed to a uniform C57BL/6 background, and B6 ES cells facilitate building complex models which combine gene inactivation with existing transgenics maintained in a B6 background. Additional phenotypic information is available at the Mouse Genome Informatics website (http://www.informatics.jax.org/external/festing/mouse/docs/C57BL.shtml). The B6 ES line was genome verified by determining the alleles at 29 SNPs that are informative across a large number of inbred mouse strains.

STRAIN: C57BL/6N

GENDER: Male

KARYOTYPE: 40XY

細胞系描述

Embryonic Stem Cells

應用

Research Category
Stem Cell Research
This Murine Embryonic Stem Cell line is derived from C57BL/6N blastocysts.

包裝

2.5 x 106 cells per vial, passage 9

外觀

Cells are supplied frozen in basal medium supplemented with 50% FCS and 10% DMSO

儲存和穩定性

Place vials in liquid nitrogen immediately upon receipt until it is convenient to proceed to subculture.

法律資訊

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Cameron Moshfegh et al.
Stem cell research, 61, 102780-102780 (2022-04-09)
Spermatogonial stem cells (SSCs) originate from gonocytes that differentiate from primordial germ cells (PGCs). In the developing mouse testis, expression of the gene LIM homeobox 1 (Lhx1) marks the most undifferentiated SSCs, which has not yet been reported for spermatogonia-like

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