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生物源
rat
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
YTS 169.4, monoclonal
物種活性
mouse
包裝
antibody small pack of 25 μg
技術
flow cytometry: suitable
immunodepletion: suitable
immunohistochemistry: suitable
同型
IgG2bκ
NCBI登錄號
UniProt登錄號
目標翻譯後修改
unmodified
基因資訊
mouse ... Cd8A(12525)
一般說明
T-cell surface glycoprotein CD8 alpha chain (UniProt: P01731; also known as T-cell surface glycoprotein Lyt-2, CD8a) is encoded by the Cd8a (also known as Lyt-2, Lyt2) gene (Gene ID: 12525) in murine species. CD8 is a single-pass type I membrane protein with one Ig-like V-type (immunoglobulin-like) domain. CD8a is produced with a 27 amino acid signal peptide that is subsequently cleaved. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.
特異性
Clone YTS 169.4 detects T-cell surface glycoprotein CD8 alpha chain (CD8a) in mouse thymocytes.
免疫原
Thymocytes from CBA mouse.
應用
Anti-CD8a, clone YTS 169.4, Cat. No. MABF1983 is a rat monoclonal antibody that detects T-cell surface glycoprotein CD8 alpha chain and has been tested for use in Flow Cytometry, Immunohistochemistry, and Immunodepletion.
Immunodepletion Analysis: A representative lot detected CD8a in Immunodepletion applications (Cobbold, S.P., et. al. (1984). Nature. 312(5994):548-51; Noort, W.A., et. al. (1996). Transpl Immunol. 4(3):198-202; Brown, C.R., et. al. (1990). J Immunol. 145(10):3438-41).
Flow Cytometry Analysis: A representative lot detected CD8a in Flow Cytometry applications (Noort, W.A., et. al. (1996). Transpl Immunol. 4(3):198-202).
Immunohistochemistry Analysis: A representative lot detected CD8a in Immunohistochemistry applications (Wright, J.R. Jr, et. al. (1997). Ann Transplant. 2(3):12-6).
Flow Cytometry Analysis: A representative lot detected CD8a in Flow Cytometry applications (Noort, W.A., et. al. (1996). Transpl Immunol. 4(3):198-202).
Immunohistochemistry Analysis: A representative lot detected CD8a in Immunohistochemistry applications (Wright, J.R. Jr, et. al. (1997). Ann Transplant. 2(3):12-6).
Research Category
Inflammation & Immunology
Inflammation & Immunology
品質
Evaluated by Flow Cytometry in mouse thymocytes.
Flow Cytometry Analysis: 1 µg of this antibody detected CD8a in mouse thymocytes.
Flow Cytometry Analysis: 1 µg of this antibody detected CD8a in mouse thymocytes.
標靶描述
27.5 kDa calculated.
外觀
Protein G purified
Format: Purified
Purified rat monoclonal antibody IgG2b in PBS without preservatives.
儲存和穩定性
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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