跳转至内容
Merck
所有图片(1)

Key Documents

MABF157B

Sigma-Aldrich

Anti-CD4 (Mouse), PerCP-Cy5.5, clone RM4-5 Antibody

clone RM4-5, from rat, PerCP-Cy5.5

别名:

T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, CD4, CD4

登录查看公司和协议定价


About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.44

生物源

rat

品質等級

共軛

PerCP-Cy5.5

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

RM4-5, monoclonal

物種活性

mouse

包裝

antibody small pack of 25 μg

技術

flow cytometry: suitable

同型

IgG2aκ

UniProt登錄號

目標翻譯後修改

unmodified

基因資訊

mouse ... Cd4(12504)

一般說明

The RM4-5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta2-domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will “compete” for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.

免疫原

Immunogen information for this antibody is proprietary information.

應用

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This rat monoclonal Anti-CD4 Antibody (Mouse), PerCP-Cy5.5, clone RM4-5, Cat. No. MABF1574 is validated for use in Flow Cytometry, for the detection of CD4.

品質

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected CD4 in one million C57Bl/6 splenocytes.

外觀

Purified by size-exclusion chromatography
Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到合适的产品?  

试试我们的产品选型工具.


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门