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Key Documents

MABD51

Sigma-Aldrich

抗-Brn-2 (POU3F2)抗体,克隆8C4.2

clone 8C4.2, from mouse

别名:

POU domain, class 3, transcription factor 2, Brain-specific homeobox/POU domain protein 2, Brain-2, Brn-2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Oct-7, Octamer-binding transcription factor 7, OTF

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

8C4.2, monoclonal

物種活性

rat, human

技術

immunohistochemistry: suitable (paraffin)
western blot: suitable

同型

IgG1κ

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... POU3F2(5454)

一般說明

Brn-2 (POU结构域,第3类,转录因子2; Oct-7; N-Oct-3; 或POU3F2)是POU 结构域转录因子大家族的成员。 高度同源的POU结构域包含N末端的一个POU特定结构域,C末端的一个POU同源结构域,以及一个互联连接子区。POU 结构域连接 DNA 序列 5’-ATGCAAAT-3’。Brn-2 在发育中的CNS中高度表达,并可能在神经形成,特别是下丘脑的发育中起一定作用。过去的研究也表明 Brn-2 可以整合 BRAF/MAP 激酶和Wnt/β-联蛋途径下游的信号,Brn-2 可能涉及黑色素瘤的转化和增殖。

免疫原

GST标记的重组蛋白,对应于人Brn-2 (POU3F2)。

應用

免疫组化分析:代表性批次的1:50稀释液可在正常大鼠脑组织、浦肯野细胞、篮细胞、大鼠小脑组织的神经胶质细胞、大鼠小脑组织的神经元、大鼠额叶皮层的神经元和神经胶质细胞以及人脑桥组织的神经元中检测Brn-2。
抗-Brn-2(POU3F2)抗体(克隆8C4.2)可检测Brn-2 (POU3F2)水平,&已发布&经验证可用于蛋白质印迹、IHC(P)。

品質

通过蛋白质印迹法在 SK-N-MC 细胞裂解液中进行了评估。

蛋白质印迹分析:该抗体的1:2,000稀释液在10 µg SK-N-MC细胞裂解液中检测到Brn-2 (POU3F2)。

標靶描述

观测值〜54 kDa。
计算分子量为47 kDa,Brn-2(POU3F2)在一些细胞裂解液中观测值为~50-55 kDa(Wolfe, A., et al., (2002).Molecular Endocrinology.16(3):435-449)。

外觀

形式:纯化

分析報告

对照
SK-N-MC细胞裂解液

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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