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生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
3A9.1, monoclonal
物種活性
human
技術
western blot: suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
ambient
目標翻譯後修改
unmodified
基因資訊
human ... PRKDC(5591)
一般說明
DNA-dependent protein kinase catalytic subunit (EC 2.7.11.1; UniProt P78527; also known as DNA-PK catalytic subunit, DNA-PKcs, DNPK1, p460) is encoded by the PRKDC (also known as HYRC, HYRC1, IMD26, XRCC7) gene (Gene ID 5591) in human. DNA-PK is a heterotrimer of PRKDC and the Ku p70/YRCC6-p86/XRCC5 dimer. It acts as a molecular sensor for DNA damage and is involved in DNA non-homologous end joining required for double-strand break (DSB) repair and V(D)J recombination. Upon binding to DNA, it expresses its catalytic properties. DNA-PKcs falls into the phosphatidylinositol (PI) 3-kinase family, however, biochemical assays indicate that it phosphorylates proteins and has no detectable activity toward lipids. DNA-PKcs is displays high similarity to PI kinase family members involved in cell cycle control, DNA repair, and DNA damage responses. Deficiency of DNA-PKcs is linked to severe combined immunodeficiency characterized by reduced or absent T and B cells, recurrent candidiasis, and lower respiratory tract infections. Ref.: Hartley, KO., et al. (1995). 82, 849-856.
特異性
Clone 3A9.1 detects DNA-PK catalytic subunit (DNA-PKcs), but not the regulatory subunit (Ku). Target epitope is present in both spliced isoforms of human DNA-PKcs reported by UniProt (P78527).
免疫原
GST-tagged recombinant fragment from the region between FAT and KIP-binding domain of human DNA-PKcs
應用
Detect DNA-PK catalytic subunit (DNA-PKcs) using this mouse monoclonal Anti-DNA-PKcs Antibody, clone 3A9.1, Cat. No. MABC1236, validated for use in Western Blotting.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
品質
Evaluated by Western Blotting in K562 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected DNA-PKcs in 10 µg of K562 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected DNA-PKcs in 10 µg of K562 cell lysate.
標靶描述
~470 kDa observed. 469.1/466.5 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).
外觀
Protein G purified.
Format: Purified
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
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