生物源
rat
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
3E2, monoclonal
物種活性
human, monkey, mouse
製造商／商標名
Chemicon®
技術
ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG2a
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... HSF2(3298)
特異性
MAB88079 recognizes a protein of 70 kDa, identified as Heat Shock Factor 2 (HSF2). The synthesis of heat shock proteins is dependent on transcriptional and post-transcriptional control in eukaryotic cells. The regulation of heat shock gene transcription is mediated by the transcriptional activator, Heat Shock Factor (HSF), which binds to heat shock response elements (HSEs). At least three HSFs have been isolated from the human, mouse, chicken, and tomato genomes and an additional factor, HSF4 has been identified in humans. HSFs purified from yeast, Drosphila, and human have different molecular weights and do not cross-react immunologically. These factors are activated by distinct stimuli. HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents whereas, the HSF2 is activated during differentiation. some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA. the phosphorylation of HSF1 may be necessary for maximal transcription of heat shock genes.
免疫原
Full length recombinant mouse HSF2 proteins (aa 1-517)
應用
Anti-Heat Shock Factor II Antibody, clone 3E2 is an antibody against Heat Shock Factor II for use in ELISA, IF, IP & WB.
Immunofluorescence Immunohistology (predominantly cytoplasmic with some nuclear). Immunoprecipitation: 2 μg/mg protein lysate. Western blot. 1:200-1:400 ELISA
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
外觀
Format: Purified
Purified immunoglobulin. Liquid containing 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.
儲存和穩定性
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
分析報告
Control
POSITIVE CONTROL:
NIH-3T3 cells
POSITIVE CONTROL:
NIH-3T3 cells
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Nitzan Levy et al.
Endocrinology, 148(7), 3449-3458 (2007-03-31)
Estrogen receptors (ERs) regulate transcription by interacting with regulatory elements in target genes. However, known ER regulatory elements cannot explain the expression profiles of genes activated by estradiol (E2) and selective estrogen receptor modulators (SERMs). We previously showed that the
Linda Pesonen et al.
Cell stress & chaperones, 26(5), 819-833 (2021-08-01)
Cancer cells rely on heat shock proteins (HSPs) for growth and survival. Especially HSP90 has multiple client proteins and plays a critical role in malignant transformation, and therefore different types of HSP90 inhibitors are being developed. The bioactive natural compound
Marek A Budzyński et al.
Molecular and cellular biology, 35(14), 2530-2540 (2015-05-13)
In mammals the stress-inducible expression of genes encoding heat shock proteins is under the control of the heat shock transcription factor 1 (HSF1). Activation of HSF1 is a multistep process, involving trimerization, acquisition of DNA-binding and transcriptional activities, which coincide
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