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生物源
mouse
品質等級
抗體表格
purified antibody
無性繁殖
1C8, monoclonal
物種活性
human
製造商／商標名
Chemicon®
技術
ELISA: suitable
immunofluorescence: suitable
western blot: suitable
同型
IgG2b
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
一般說明
Erythropoietin (Epo) is a glycoprotein consisting of one 165-amino acid single-chain polypeptide with two internal disulfide bonds. When fully glycosylated, the molecular weight of Epo is approximately 34 kDa. Normally produced by the liver and kidney, Epo is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia.
特異性
Reacts with human Erythropoietin (Epo).
免疫原
Recombinant human Epo.
應用
Detect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.
ELISA:
For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate.
Immunosorbent preparation
Optimal working dilutions must be determined by the end user.
For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate.
Immunosorbent preparation
Optimal working dilutions must be determined by the end user.
Research Category
Metabolism
Metabolism
Research Sub Category
Metabolic Hormones & Receptors
Metabolic Hormones & Receptors
品質
Evaluated by Western Blot on Mouse kidney lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.
標靶描述
34 kDa (glycosylated)
外觀
Protein A purified
Format: Purified
Purified mouse monoclonal IgG2b in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
儲存和穩定性
Stable for 1 year at 2-8ºC from date of receipt.
分析報告
Control
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Thomas H Sanderson et al.
PloS one, 8(11), e78627-e78627 (2013-11-14)
Recent advancements in isolation techniques for cytochrome c (Cytc) have allowed us to discover post-translational modifications of this protein. We previously identified two distinct tyrosine phosphorylated residues on Cytc in mammalian liver and heart that alter its electron transfer kinetics
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