推荐产品
生物来源
human
质量水平
重组
expressed in CHO cells
方案
≥95% (one N-glycosylation site)
表单
liquid
比活
3.99 x 108 U/mg
制造商/商品名称
Chemicon®
浓度
1 x 106 units/mL
技术
cell culture | mammalian: suitable
NCBI登记号
UniProt登记号
运输
dry ice
相关类别
一般描述
产品来源:从CHO细胞中表达的人成纤维细胞mRNA中获得的cDNA。
重组人干扰素β 1a (Hu-IFNβ 1a)。 分子量约为 23 kDa。
联系
替代:IF008
外形
50 mM NaOAc,pH 5.5,含有 0.1% BSA中的冷冻液体。
储存及稳定性
接收后,该产品应在-70°℃或更低温度下保存,以保留全部活性。
用冷水孵育解冻产品瓶,直到刚解冻。解冻的IFNβ应
以工作等分试样保存于-70°C中,可保存长达六个月。避免多次冻融循环。
再冻结应在干冰或干冰/酒精浴中进行。
产品的进一步稀释应在含有蛋白质的缓冲液中进行,例如 0.1% 牛血清白蛋白 (BSA)。
用冷水孵育解冻产品瓶,直到刚解冻。解冻的IFNβ应
以工作等分试样保存于-70°C中,可保存长达六个月。避免多次冻融循环。
再冻结应在干冰或干冰/酒精浴中进行。
产品的进一步稀释应在含有蛋白质的缓冲液中进行,例如 0.1% 牛血清白蛋白 (BSA)。
分析说明
比活度:通过检测病毒诱导的细胞病变的抑制作用来确定。在该干扰素抗病毒试验中,大约1单位/ml的干扰素是产生50%细胞病变效应所必需的量。这些单位是根据美国国立卫生研究院提供的人干扰素β(Hu-IFNβ)国际参考标准确定的。使用水泡性口炎病毒 (VSV) 测量 VERO 细胞的活性单位。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
其他客户在看
Fanning Zeng et al.
Cells, 9(12) (2020-12-10)
Genetic knock-in using homology-directed repair is an inefficient process, requiring the selection of few modified cells and hindering its application to primary cells. Here, we describe Homology independent gene Tagging (HiTag), a method to tag a protein of interest by
Colton R Martens et al.
PLoS pathogens, 18(2), e1010342-e1010342 (2022-02-23)
Viral infection of the heart is a common but underappreciated cause of heart failure. Viruses can cause direct cardiac damage by lysing infected cardiomyocytes. Inflammatory immune responses that limit viral replication can also indirectly cause damage during infection, making regulatory
Sarah Aref et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 28(4), 1043-1055 (2020-02-23)
The mechanism of tumor-selective replication of oncolytic measles virus (MV) is poorly understood. Using a stepwise model of cellular transformation, in which oncogenic hits were additively expressed in human bone marrow-derived mesenchymal stromal cells, we show that MV-induced oncolysis increased
Zhaowei Chu et al.
Nature communications, 13(1), 1859-1859 (2022-04-08)
The cohesin complex participates in the organization of 3D genome through generating and maintaining DNA loops. Stromal antigen 2 (STAG2), a core subunit of the cohesin complex, is frequently mutated in various cancers. However, the impact of STAG2 inactivation on
Daniel Vogt et al.
Virology, 540, 150-159 (2020-01-14)
During DNA virus infections, detection of cytosolic DNA by the cGAS-STING pathway leads to activation of IFN-β. Kaposi's Sarcoma Herpesvirus (KSHV), an oncogenic DNA virus, is the etiological agent of Kaposi's Sarcoma, an endothelial cell (EC)-based tumor. To investigate the
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门