推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
225, monoclonal
形狀
lyophilized
不包含
preservative
物種活性
human
應無反應活性
rat, mouse
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
同型
IgG1
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)
一般說明
Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.
Recognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells.
免疫原
Human
partially purified, human EGFR from A431 cells
應用
Immunoprecipitation (1-2 µg per sample)
Neutralization Studies (2 µg/ml, see comments and application references)
Neutralization Studies (2 µg/ml, see comments and application references)
警告
Toxicity: Standard Handling (A)
外觀
Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.
重構
Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
分析報告
Negative Control
HL-60 cells
HL-60 cells
Positive Control
A431 cells
A431 cells
其他說明
Hunter T. 1984. Nature311, 414.
Reynolds, F.H., et. al 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Reynolds, F.H., et. al 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Anguo Liu et al.
The Journal of biological chemistry, 284(10), 6389-6402 (2009-01-09)
Thrombospondin (TSP) 1 is a trimeric multidomain protein that contains motifs that recognize distinct host cell receptors coupled to multiple signaling pathways. Selected TSP1-induced cellular responses are tyrosine kinase-dependent, and TSP1 contains epidermal growth factor (EGF)-like repeats. Specific receptor interactions
Pallavi Garg et al.
American journal of physiology. Lung cellular and molecular physiology, 301(1), L79-L90 (2011-05-03)
Thrombospondin-1 (TSP1) is a multidomain protein that contains epidermal growth factor (EGF)-like repeats that indirectly activate the EGF receptor (EGFR) and selected downstream signaling pathways. In these studies, we show that TSP1 opens the paracellular pathway in human lung microvascular
Caren C Bancroft et al.
International journal of cancer, 99(4), 538-548 (2002-05-07)
We previously reported that expression of angiogenesis factors interleukin-8 (IL-8) and vascular endothelial growth factor (VEGF) is promoted by coactivation of transcription factors nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1) by interleukin-1alpha in human head and neck squamous cell carcinomas
Silvia Ancona et al.
Biomedicines, 9(12) (2021-12-25)
Matrix metalloproteinase (MMP) dysregulation is implicated in several diseases, given their involvement in extracellular matrix degradation and cell motility. In lymphangioleiomyomatosis (LAM), a pulmonary rare disease, MMP-2 and MMP-9 have been detected at high levels in serum and urine. LAM
Jiarui Bi et al.
Journal of cell science, 133(5) (2019-11-15)
In periodontal disease (PD), bacterial biofilms cause gingival inflammation, leading to bone loss. In healthy individuals, αvβ6 integrin in junctional epithelium maintains anti-inflammatory transforming growth factor-β1 (TGF-β1) signaling, whereas its expression is lost in individuals with PD. Bacterial biofilms suppress
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