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CP18

Sigma-Aldrich

抗胶原II型(Ab-1)小鼠mAb(II-4C11)

liquid, clone II-4C11, Calbiochem®

别名:

Collagen Type II antibody

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.43

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

II-4C11, monoclonal

形狀

liquid

包含

≤0.1% sodium azide as preservative

物種活性

rat, human, bovine, rabbit

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
avoid repeated freeze/thaw cycles

同型

IgG1

運輸包裝

wet ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... COL2A1(1280)

一般說明

可识别约130 kDa的II型胶原蛋白。可能与其他胶原发生反应。当以高浓度使用时,也可以检测到对应于聚集体的更高条带。
经验证,该抗II型胶原(Ab-1)小鼠mAb(II-4C11)可用于冰冻切片检测II型胶原(Ab-1)。
通过用指定的免疫原免疫BALB/c小鼠并将脾细胞与P3-X-63-Ag8-LUI小鼠骨髓瘤融合而产生的纯化的小鼠单克隆抗体。可识别约130 kDa的II型胶原蛋白。

免疫原

纯化的II型胶原

應用

冷冻切片(1-10 µg/ml)

免疫印迹(1-5 µg/ml,请参阅应用参考)

石蜡切片(1-10 µg/ml,请参阅应用参考)

包裝

请参考特定浓度批号的标签。

警告

毒性:标准处理(A)

外觀

溶于100 mM磷酸钠缓冲液,0.1% BSA(pH 7.0)。

重構

初次融化后,等分并冷冻(-20°C)。

其他說明

Kumagai, J. et al. 1994 J. Anat.185, 278.
Kivrikko, K,I.1993 Ann.Med.25, 113.
Sandell, L.J., et al. 1991 J. Cell.Biol.114, 1307.
Ryan, M.C., and Sandell, L.J.1990 J. Biol. Chem.265, 10334.
Mayne, R., and Burgeson, R.E.1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
可能与其他胶原发生反应。当以高浓度使用时,也可以检测到对应于聚集体的更高条带。该抗体也可以在高于1 µg/mL的浓度下与其他类型的胶原反应。在免疫印迹中,获得了约130 kDa的主要条带。在单个系统中,应对抗体进行滴定以获得最佳结果。

法律資訊

由Daiichi Fine Chemical Co.,Ltd.生产,未在日本销售。
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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