推荐产品
生物源
bovine
品質等級
化驗
>95% (total protein)
形狀
liquid
製造商／商標名
Chemicon®
技術
cell based assay: suitable
UniProt登錄號
運輸包裝
dry ice
基因資訊
bovine (calf) ... PRNP(281427)
一般說明
Histidine-tagged full-length mature part of bovine PrP (25-244) is expressed in E. Coli BL21, solubilized from inclusion bodies in 6 M guanidine-HCl, and purified by Ni(II)-nitriloacetate agarose chromatography followed by reversed-phase HPLC (C4 column)
Prion diseases or transmissible spongiform encephalopathies are neurodegenerative diseases that affect both humans and animals (Prusiner 1998). All prion diseases share the same molecular pathogenic mechanism that involves conversion of normal cellular prion protein (PrPc) into a form that is insoluble in non ionic detergent and partially resistant to proteases (PrPSc) (Pan et al. 1993). Both PrPSc and PrPc are encoded within a single exon of a chromosomal gene as a protein of ~ 250 amino acids (Basler et al. 1986). Many mammalian PrPs have a 22 amino acid N-terminal signal sequence (Hope et al. 1986; Turk et al. 1988) and 23 amino acid C-terminal signal sequence encoding for attachment of a glycosylphosphatidylinositol anchor (Stahl et al. 1987, 1990). The mature protein of 209 amino acids contains one disulfide bond (Turk et al. 1988) and has two sites of asparagine-linked glycosylation (Endo et al. 1989; Oesch et al. 1995).
應用
Antigen in standard immunochemical detection of BSE.
Optimal working dilution must be determined by the end user.
Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
外觀
Liquid in 10 mM sodium acetate buffer, pH 4.0, containing 0.01% sodium azide.
儲存和穩定性
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
分析報告
The PrPc appears as a single band of about 27 kD by SDS-PAGE
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Scrapie and cellular PrP isoforms are encoded by the same chromosomal gene.
Basler, K, et al.
Cell, 46, 417-428 (1986)
Purification and properties of the cellular and scrapie hamster prion proteins.
Turk, E, et al.
European Journal of Biochemistry, 176, 21-30 (1988)
The major polypeptide of scrapie-associated fibrils (SAF) has the same size, charge distribution and N-terminal protein sequence as predicted for the normal brain protein (PrP).
Hope, J, et al.
The Embo Journal, 5, 2591-2597 (1986)
Identification of glycoinositol phospholipid linked and truncated forms of the scrapie prion protein.
Stahl, N, et al.
Biochemistry, 29, 8879-8884 (1990)
Prions.
Prusiner, S B
Proceedings of the National Academy of Sciences of the USA, 95, 13363-13383 (1998)
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