推荐产品
生物源
rabbit
品質等級
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
human
技術
immunocytochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... ADAM17(6868)
一般說明
ADAM17, also known as TNF-alpha Convertase, or TACE, was first cloned from human epithelial cells. Tumor-necrosis factor alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-alpha is synthesized as a 26 kDa Type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-alpha. TNF-alpha converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. The ADAM proteins are structurally similar, with a signal sequence, metalloprotease domain (inactive in some ADAMs), disintegrin domain, cystein rich domain, EGF like repeat, type I transmembrane, and a cytoplasmic domain. A member of the metalloproteinase family containing disintegrin like domains (ADAMs), ADAM17 is known to process the TNF alpha trimer from the membrane attached precursor to the soluble form. ADAM17 contains the canonical HExxHxxxxxH zinc metallo-proteinase motif, and has been shown to be proteolytically active, cleaving TNF precursor as well as TNF p75 receptor, myeloid precursor protein, amyloid plaque protein, L selectin and TGF alpha, making ADAM17 a "sheddase". Reports have shown that ADAM17 expression in human breast tumor samples is an indicator of poor prognosis.
特異性
This antibody recognizes the cytoplasmic domain of Adam17.
免疫原
Epitope: Cytoplasmic domain
GST-tagged recombinant protein corresponding to the cytoplasmic domain of mouse Adam17.
應用
Anti-Adam17 Antibody is a highly specific rabbit polyclonal antibody, that targets ADAM 17 & has been tested in western blotting & ICC.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Adam17 in 10 µg of the following cell lysates--C2C12, HEK293, HeLa, HepG2, HUVEC, L6, NIH/3T3, PC12, and PC3.
Western Blotting Analysis: A representative lot detected pro-form and the truncated, mature form of Adam17 in COS cell lysates (Schlondorff, J., et al. (2000) Biochem J. 1(347):131-138.).
Western Blotting Analysis: A representative lot detected Adam17 in COS-7 cell lysates (Zheng, Y., et al. (2002). J Biol Chem. 277(45):42463-42470.).
Western Blotting Analysis: A representative lot detected the Pro-form and the truncated. mature form of Adam17 in COS-7 cell lysates (Le Gall, S. M., et al. (2010). J Cell Sci. 123(22):3913-3922.).
Western Blotting Analysis: A representative lot detected pro-form and the truncated, mature form of Adam17 in COS cell lysates (Schlondorff, J., et al. (2000) Biochem J. 1(347):131-138.).
Western Blotting Analysis: A representative lot detected Adam17 in COS-7 cell lysates (Zheng, Y., et al. (2002). J Biol Chem. 277(45):42463-42470.).
Western Blotting Analysis: A representative lot detected the Pro-form and the truncated. mature form of Adam17 in COS-7 cell lysates (Le Gall, S. M., et al. (2010). J Cell Sci. 123(22):3913-3922.).
品質
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Adam17 in 10 µg of A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Adam17 in 10 µg of A431 cell lysate.
標靶描述
~120 kDa and ~100 kDa observed. The pro-form and the truncated, mature form of Adam17 may be observed at 120 kDa and 100 kDa, respectively (Schlondorff, J., et al. (2000) Biochem J. 1(347):131-138.; Le Gall, S. M., et al. (2010). J Cell Sci. 123(22):3913-3922.).
外觀
Unpurified
Rabbit polyclonal serum with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析報告
Control
A431 cell lysate
A431 cell lysate
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
Pinchas Tsukerman et al.
Oncotarget, 6(42), 44151-44160 (2015-12-20)
Genetic deficiencies provide insights into gene function in humans. Here we describe a patient with a very rare genetic deficiency of ADAM17. We show that the patient's PBMCs had impaired cytokine secretion in response to LPS stimulation, correlating with the
Wei Wang et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(10), 13935-13948 (2020-08-28)
Epithelial ovarian carcinoma tissues express high levels of tumor necrosis factor-alpha (TNF-α) and other inflammatory cytokines. The underlying mechanism leading to the abnormal TNF-α expression in ovarian cancer remains poorly understood. In the current study, we demonstrated that lysophosphatidic acid
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