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Merck
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Key Documents

ABT283

Sigma-Aldrich

Anti-Cadherin-11/OB Cadherin Antibody

from rabbit, purified by affinity chromatography

别名:

Cadherin-11, OSF-4, Osteoblast cadherin, OB-cadherin

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

mouse, human

物種活性(以同源性預測)

rat (based on 100% sequence homology)

技術

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... CDH11(1009)

一般說明

Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.

免疫原

Epitope: Cadherin 5 domain
KLH-conjugated linear peptide corresponding to the Cadherin 5 domain of Human Cadherin-11/OB Cadherin.

應用

Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
This Anti-Cadherin-11/OB Cadherin Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Cadherin-11/OB Cadherin.
Western Blotting Analysis: 2 µg/mL of a representative lot of this antibody detected Cadherin-11/OB Cadherin in 10 µg of Mouse Brain, Mouse Brain Cytosol, Mouse Brain Membrane, Mouse Brain Nuclear Extract, Mouse (Aged) Brain Frontal Cortex, and Mouse Cerebellum tissue lysate.

Immunohistochemistry Analysis: A 1:50 dilution of a representative lot of this antibody detected Cadherin-11/OB Cadherin in neurons of the human cerebral cortex and thalamus, as well as purkinje and basket cells in human cerebellum.

品質

Evaluated by Western Blotting in Human Thalamus Tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Cadherin-11/OB Cadherin in 10 µg of Human Thalamus Tissue lysate.

標靶描述

~75 kDa observed.
Full length protein has a molecular weight of ~120 kDa, however the cleaved product has a molecular weight of ~75-80 kDa (McCusker, C., et al. (2009) MBC. 20(1):78-89). Uncharacterized bands may be observed in some lysates.

外觀

Antigen Affinity Purified
Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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