推荐产品
生物源
rabbit
品質等級
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
mouse, rat, human
技術
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... TBX5(6910)
一般說明
The gene encoding T-box transcription factor 5 (TBX5) is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity.
免疫原
Recombinant protein corresponding to mouse T-box transcription factor TBX5.
應用
Anti-T-box transcription factor TBX5 Antibody detects level of T-box transcription factor TBX5 & has been published & validated for use in Western Blotting, ICC & IHC.
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected T-box transcription factor TBX5 in 293T cells overexpressing T-box transcription factor TBX5, mouse primary cardiocytes treated with serum, HA-tagged Tbx5 transfected NIH/3T3 cells, and HA-tagged Tbx5 transfected C2C12 cells (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.)
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected T-box transcription factor TBX5 in mouse embryonic tissue and in Tbx5 transgenic mouse heart tissues treated with tamoxifin (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected T-box transcription factor TBX5 in mouse embryonic tissue and in Tbx5 transgenic mouse heart tissues treated with tamoxifin (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
品質
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected T-box transcription factor TBX5 in 10 µg of HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected T-box transcription factor TBX5 in 10 µg of HeLa cell lysate.
標靶描述
~53 kDa observed. An uncharacterized band may be observed at ~75 kDa in some cell lysates. Three isoforms due to alternative splicing may be observed at ~35 kDa, ~50 kDa, and ~64 kDa in some cell lysates. (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.).
外觀
Unpurified
Rabbit polyclonal serum with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析報告
Control
HeLa cell lysate
HeLa cell lysate
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
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