推荐产品
生物源
rabbit
品質等級
抗體表格
affinity purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
bovine, mouse, rat
製造商／商標名
Chemicon®
技術
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
mouse ... Nos1(18125)
特異性
Reactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse.
免疫原
Synthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH.
應用
Anti-Nitric Oxide Synthase I Antibody is an antibody against Nitric Oxide Synthase I for use in ELISA, WB, IH.
Immunohistochemistry: Use at 5 - 10 μg/mL on rat neuronal sections fixed with 3.5% paraformaldehyde. Incubation overnight at 4°C with a 1:100 - 1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons.
Western blot: 1 - 10 μg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155 kDa protein.
ELISA: Use at 1.0 μg/mL or lower on plates coated with 50 - 100 ng of peptide antigen.
Optimal working dilutions must be determined by the end user.
Western blot: 1 - 10 μg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155 kDa protein.
ELISA: Use at 1.0 μg/mL or lower on plates coated with 50 - 100 ng of peptide antigen.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Oxidative Stress
Oxidative Stress
外觀
Peptide affinity-purified immunoglobulin. Supplied at a 1mg/mL solution in PBS, pH 7.4, containing 0.1% BSA.
儲存和穩定性
Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated thawing and refreezing.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
A Özen et al.
Anatomia, histologia, embryologia, 42(5), 384-393 (2013-02-16)
This study was aimed to determine staining intensity, cellular localization and distribution of the nitric oxide synthase (NOS) enzymes during the sexual cycle in the cow oviduct. Oviduct samples belonging to 20 cows, 10 of which were in the estrual
Agustín España et al.
Experimental dermatology, 22(2), 125-130 (2013-02-01)
Pemphigus vulgaris (PV) is an autoimmune blistering skin disease characterized by suprabasal acantholysis produced as a consequence of desmoglein (Dsg) and non-Dsg autoantibodies binding to several targeting molecules localized on the membrane of keratinocytes. Nitric oxide (NO) may exert a
G Scala et al.
Anatomia, histologia, embryologia, 41(6), 428-435 (2012-04-18)
The reticular groove mucosa of adult cattle, buffalo and sheep was investigated by histochemical and immunocytochemical techniques. Intense NADPH-d staining was observed in the folds of the epithelium mucosa and at the bottom of the reticular groove in all domestic
Gaetano Scala et al.
Anatomical record (Hoboken, N.J. : 2007), 290(11), 1399-1412 (2007-10-12)
Choroid plexuses (CPs) play pivotal roles in many processes that establish, survey, and maintain the biochemical and cellular status of the central nervous system (CNS). Changes in the anatomy and physiology of CPs have been linked to several CNS diseases.
Mark R Griffiths et al.
Immunology, 155(3), 346-355 (2018-06-21)
Microglia and non-professional immune cells (endothelial cells, neurons) participate in the recognition and removal of pathogens and tissue debris in the injured central nervous system through major pro-inflammatory processes. However, the mechanisms involved in regulating these responses remain ill-characterized. We
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