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Merck
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文件

537060

Sigma-Aldrich

Propidium Iodide Solution

A convenient form of propidium iodide useful for flow cytometry studies.

别名:

Propidium Iodide Solution

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About This Item

经验公式(希尔记法):
C27H34I2N4
CAS号:
分子量:
668.39
MDL號碼:
分類程式碼代碼:
12352200
NACRES:
NA.47

品質等級

形狀

liquid

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
protect from light

輸入

sample type intact cells

螢光

λex 536 nm
λem 617 nm

運輸包裝

ambient

儲存溫度

2-8°C

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChI 密鑰

XJMOSONTPMZWPB-UHFFFAOYSA-M

相关类别

一般說明

Membrane impermeant DNA intercalator. Useful for staining apoptotic cells and nuclei by flow cytometry. Useful in differentiating between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes, e.g. fluorescein isothiocyanate. Excitation max.: ~536 nm; Emission max.: ~617 nm.

生化/生理作用

Cell permeable: no
Primary Target
Membrane impermeable DNA intercalator
Product does not compete with ATP.
Reversible: no

警告

Toxicity: Standard Handling (A)

外觀

50 µg/ml in PBS, pH 7.4.

重構

Following initial use, aliquot and freeze (-20°C).

其他說明

Belloc, F., et al. 1994. Cytometry17, 59.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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实验方案

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Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

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