推荐产品
物種活性
human, mouse, rat
品質等級
製造商／商標名
Milliplex®
技術
multiplexing: suitable
檢測方法
fluorometric (Luminex® xMAP®)
儲存溫度
2-8°C
一般說明
The MILLIPLEX® Total/Phospho Akt (48-618MAG) and Total/Phospho ERK (Catalog No. 48-619) Magnetic bead kits have been developed for the simultaneous detection of phosphorylated Akt (Ser473) and total Akt or phosphorylated ERK1/2 (Thr185/Tyr187) and total ERK in a single well using the Luminex system. These detection assays are rapid, convenient alternatives to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples.
特異性
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
應用
Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples. An overnight (4°C) incubation is recommended for best results.This assay requires 25 μL diluted cell lysate per well.This kit must be run using Assay Buffer 2 (provided).1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL)Analytes available:Akt (Total)Akt (Ser473)
包裝
96-well plate
法律資訊
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
訊號詞
Danger
危險分類
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
儲存類別代碼
10 - Combustible liquids
相关内容
MILLIPLEX® cell signaling assays facilitate detection of phosphoproteins and total proteins in cellular pathways.
MILLIPLEX® cell signaling assays facilitate detection of phosphoproteins and total proteins in cellular pathways.
MILLIPLEX® cell signaling assays facilitate detection of phosphoproteins and total proteins in cellular pathways.
MILLIPLEX® cell signaling assays facilitate detection of phosphoproteins and total proteins in cellular pathways.
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