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product name
EGF，人，重组，大肠埃希菌,
重組細胞
expressed in E. coli
品質等級
化驗
≥95% (SDS-PAGE)
形狀
solid
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
雜質
<1.0 EU/μg Endotoxin (EU/μg EGF)
運輸包裝
ambient
儲存溫度
−20°C
一般說明
在大肠埃希菌中表达的重组人表皮生长因子（54个氨基酸）。 除了一个额外的N端蛋氨酸，与天然人EGF相同。人EGF与小鼠EGF同源性为70%，并已显示具有相似的生物学活性。对来自组织（例如乳房，角膜，表皮，真皮，肝脏，胰腺，神经，羊膜和肾上腺髓质）的多种外胚层和内胚层来源的细胞具有促有丝分裂作用。EGF与其170 kDa跨膜受体的结合导致受体的二聚化，内在受体酪氨酸激酶的激活以及导致细胞增殖和/或分化的复杂信号转导级联反应的启动。用作在预防细胞凋亡中的存活因子。
在大肠埃希菌中表达的重组人表皮生长因子（54个氨基酸）。除了一个额外的N端蛋氨酸，与人EGF相同。人EGF与小鼠EGF同源性为70%，并已显示具有相似的生物学活性。对来自组织（例如乳房，角膜，表皮，真皮，肝脏，胰腺，神经，羊膜和肾上腺髓质）的多种外胚层和内胚层来源的细胞具有促有丝分裂作用。用作在预防细胞凋亡中的存活因子。
生化／生理作用
ED₅₀ = 100-400 pg/ml，通过其刺激EGF反应性小鼠成纤维细胞系Balb/3T3中³H-胸苷掺入的能力来衡量。
警告
毒性：标准处理（A）
重構
复溶后，等分并冷冻长期保存（-20°C或-70°C），或冷藏（4°C）短期保存。避免溶液反复冻融。储备溶液在4°C下可稳定保存1个月，在-20°C或-70°C下可稳定保存3个月。
用含有≥0.1％HSA或BSA的10 mM乙酸复溶成浓度≥10 µg/ml的溶液。
其他說明
Merlo, G.R., et al. 1995.J. Cell Biol. 128, 1185.
Yoshida, T., et al. 1993.Brain Res. Dev.76, 147.
Rubin, J.S., et al. 1991.Proc.Natl.Acad.Sci. USA88, 415.
Carpenter, G., and Wahl, M.I.1990.In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.)Springer-Verlag, New York, p 69.
Downward, J., et al. 1984.Nature311, 483.
Gray, A., et al. 1983.Nature303, 722.
Scott, J., et al. 1983.Science221, 236.
Cohen, S. 1960.Proc.Natl.Acad.Sci. USA46, 302.
Yoshida, T., et al. 1993.Brain Res. Dev.76, 147.
Rubin, J.S., et al. 1991.Proc.Natl.Acad.Sci. USA88, 415.
Carpenter, G., and Wahl, M.I.1990.In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.)Springer-Verlag, New York, p 69.
Downward, J., et al. 1984.Nature311, 483.
Gray, A., et al. 1983.Nature303, 722.
Scott, J., et al. 1983.Science221, 236.
Cohen, S. 1960.Proc.Natl.Acad.Sci. USA46, 302.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
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