219360
Cathepsin D/E Substrate, Fluorogenic
An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E, but not cathepsins B, H, or L .
别名:
Cathepsin D/E Substrate, Fluorogenic, MOCAc-GKPILF~FRLK(Dnp)- D-R-NH₂, MOCAc-GKPILF~FRLK(Dnp)-D-R-NH₂
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About This Item
推荐产品
品質等級
化驗
≥97% (HPLC)
形狀
lyophilized solid
製造商/商標名
Calbiochem®
儲存條件
OK to freeze
desiccated (hygroscopic)
protect from light
顏色
yellow
溶解度
DMSO: 5 mg/mL
螢光
λex ~328 nm
λem ~393 nm
運輸包裝
ambient
儲存溫度
2-8°C
一般說明
An internally quenched fluorogenic 11 amino acid peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively). Does not act as a substrate for cathepsins B, H, or L. Cleavage occurs at the Phe-Phe amide bond resulting in enhanced fluorescence. Useful for screening cathepsin D and E inhibitors and for determining cathepsin D and E activity in tissue and cell extracts. Excitation max: ~328 nm; emission max: ~393 nm.
An internally quenched fluorogenic 11-mer peptide that acts as a sensitive and selective substrate for cathepsins D and E (kcat/Km = 15.6 µM-1s-1 and 10.9 µM-1s-1 for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively), but not cathepsins B, H, or L . Cleavage occurs between Phe-Phe residues and results in enhanced fluorescence. A useful tool for inhibitors screening or for the determination of cathepsins D and E activities in crude extracts of various tissues and cell lysates.
生化/生理作用
Cell permeable: no
Primary Target
Substrate for cathepsins D and E
Substrate for cathepsins D and E
Product does not compete with ATP.
Reversible: no
kcat/Km = 15.6 µM⁻¹ sec⁻¹ and 10.9 µM⁻¹ sec⁻¹ for rat gastric cathepsin D and human erythrocyte cathepsin E, respectively
包裝
Packaged under inert gas
警告
Toxicity: Standard Handling (A)
序列
MOCAc-Gly-Lys-Pro-Ile-Leu-Phe~Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH₂
MOCAc = (7-Methoxycoumarin-4-yl)acetyl
MOCAc = (7-Methoxycoumarin-4-yl)acetyl
外觀
Supplied as a trifluoroacetate salt.
重構
Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
其他說明
Yasuda, Y., et al. 1999. J. Biochem.126, 260.
Yasuda, Y., et al. 1999. J. Biochem.125, 1137.
Yasuda, Y., et al. 1999. J. Biochem.125, 1137.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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