14-484-M

PKCα Protein, active, 10 µg

Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.

• 别名: PRKCA, Protein kinase C alpha type
• 分類程式碼代碼: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

属性

• 生物源: human
• 品質等級: 100
• 重組細胞: expressed in baculovirus infected Sf21 cells
• 產品線: Upstate^®
• 比活性: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• 儲存期限: 1 yr
• 分子量: M_w 78 kDa
• 純化經由: (Ni²⁺/NTA-agarose)
• 物種活性: human
• 製造商／商標名: Upstate^®
• 技術: activity assay: suitable (kinase)
• 登錄號: (FUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. ); NM_002737.2
• NCBI登錄號: NM_002737.2
• UniProt登錄號: P17252
• 儲存溫度: −70°C
• 基因資訊: human ... PRKCA(5578)

说明

一般說明

Research area: Apoptosis

Protein Target: PKCα

Target Sub-Family: AGC

Recombinant, full-length human PKCα, containing N-terminal His6 tag

應用

Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.

生化／生理作用

Protein kinase C-α (PKCα), an isoform of protein kinase C, exhibits an anti-apoptotic and pro-proliferative function. It is commonly upregulated in tumor cells, and its depletion has been associated with apoptosis induction. The anti-apoptotic effects of PKCα are mediated through mechanisms such as phosphorylation of the anti-apoptotic protein B-cell lymphoma 2 (Bcl-2) and activation of the pro-survival protein kinase AKT. The inhibition of PKCα leads to the activation of a PKCδ-dependent apoptotic pathway in salivary epithelial cells. PKCα regulates cell cycle progression in glioma cells by increasing the expression of p21 cyclin-dependent kinase inhibitor 1 (p21 (Waf1/Cip1)), a protein involved in cell cycle regulation.

包裝

Also available in 10 μg size and in bulk

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-484M when ordering the 250 µg size.

品質

routinely evaluated by phosphorylation of Histone H1.

標靶描述

PKCα

外觀

Ni²⁺/NTA-agarose

儲存和穩定性

1 year at -70°C

其他說明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H317
• 防範說明: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危險分類: Skin Sens. 1
• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°C）: Not applicable