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Merck
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Key Documents

05-1592

Sigma-Aldrich

抗-mTOR抗体，克隆21D8.2

clone 2ID8.2, from mouse

别名:

FK506 binding protein 12-rapamycin associated protein 1, FK506 binding protein 12-rapamycin associated protein 2, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP-rapamycin associated protein, FKBP12-rapamycin complex-associated prot

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

100

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

2ID8.2, monoclonal

物種活性

mouse, human

技術

immunocytochemistry: suitable
western blot: suitable

同型

IgG1κ

NCBI登錄號

NP_004949.1

UniProt登錄號

P42345

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... MTOR(2475)

一般說明

mTOR（雷帕霉素的哺乳动物靶标，又名 FRAP、RAPT 或 RAFT）是 289kDa 的 Ser/Thr 蛋白激酶，可调节细胞周期进程、细胞生长、蛋白合成、核糖体生物合成和自噬。mTOR 是磷酸肌醇激酶相关激酶（PIKK）家族的进化保守成员，其活性通过响应胰岛素或肌肉活性，由 Akt 在 Ser2448 上的磷酸化来调节。 mTORC1（mTOR复合物 1）至少由mTOR、Raptor和G（mLST8）组成。众所周知，mTORC1在胰岛素信号传导中发挥核心作用，而胰岛素信号传导对维持代谢平衡至关重要。mTORC2主要通过使Ser473上的疏水基团Akt（PI3激酶通路的著名效应器）磷酸化来影响细胞的增殖和存活。除了磷酸化 Akt 外，还已知 mTORC2 复合物通过 Rac、Rho 和 PKC 发挥其作用，影响细胞骨架的组织和迁移。两种 mTOR 复合物中的缺失都与多种疾病有关，包括癌症和糖尿病。

特異性

该抗体可识别mTOR。

免疫原

Flag标记全长mTOR蛋白

應用

免疫细胞化学分析：该抗体先前批次的1:500稀释液在C2C12、HeLa和NIH/3T3细胞裂解液中检测到mTOR。
抗-mTOR抗体，克隆21D8.2是用于WB&IP的抗mTOR抗体。
研究子类别
PI3K、Akt & mTOR 信号传导
研究类别
信号传导

品質

通过蛋白质印迹对HEK293细胞裂解物中的抗体进行了评估。
蛋白质印迹分析：0.5 µg/mL的该抗体在HEK293细胞裂解液中检测到mTOR。

標靶描述

289kda

聯結

替代：04-385

外觀

形式：纯化
纯化免疫球蛋白，含有0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠。
蛋白G纯化

儲存和穩定性

自接收之日起，在2-8°C下冷藏保存1年。

分析報告

对照
Hek293细胞裂解液

其他說明

浓度：请参考批次特异性浓缩物的检验报告。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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