推荐产品
生物源
rabbit
品質等級
抗體表格
culture supernatant
抗體產品種類
primary antibodies
無性繁殖
DW2, monoclonal
物種活性
vertebrates (common species)
技術
western blot: suitable
同型
IgG
運輸包裝
dry ice
目標翻譯後修改
unmodified
一般說明
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule that corresponds to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
特異性
Reactivity with other species has not been confirmed.
Recognizes recombinant proteins containing the HA epitope tag. The apparent molecular weight is related to the tagged protein.
免疫原
KLH-conjugated, synthetic peptide containing the sequence …CYPYDVPDY… derived from the influenza virus hemaglutinin (HA) protein.
應用
Anti-HA Tag Antibody, clone DW2 is an antibody against HA Tag for use in WB.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Western Blot Analysis: A 1:1,000-1:2,000 dilution of this lot detected HA-tagged WNT-1 protein expressed in HEK293 cells.
品質
Western Blot Analysis
Lysates from non-transfected (lane 1) and HAtagged WNT-1 transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HA-Tag, clone DW2 (1:1,000 dilution).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates the HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.
Lysates from non-transfected (lane 1) and HAtagged WNT-1 transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HA-Tag, clone DW2 (1:1,000 dilution).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates the HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.
標靶描述
Various
聯結
Replaces: 05-902
外觀
Cultured supernatant in 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析報告
Control
HA-tagged WNT-1 protein.
HA-tagged WNT-1 protein.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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